文冠果油脂的水酶法提取及生物柴油制备
文献类型：学位论文
| 作者 | 刘苗苗 |
| 学位类别 | 硕士 |
| 答辩日期 | 2007-06-03 |
| 授予单位 | 中国科学院过程工程研究所 |
| 授予地点 | 过程工程研究所 |
| 导师 | 王玉春 |
| 关键词 | 文冠果 水酶法 生物柴油 气相色谱 |
| 其他题名 | Oil Extraction from Xanthoceras sorbifolia Bunge Seed by Aqueous Enzymatic Method and Biodiesel Preparation |
| 学位专业 | 生物化工 |
| 中文摘要 | 生物柴油作为一种清洁能源，具有安全、环保和可再生的特点，可以作为石化柴油很好的替代能源。在我国制约生物柴油发展的主要问题是原料来源及成本问题，寻找优质的生物柴油原料是解决其发展的重要途径。木本油料植物文冠果可在贫瘠土地种植，种子含油率高，以其为原料生产生物柴油具有重要的实用价值。 本论文通过单因素实验和响应面法分别优化了水酶法提取文冠果油脂和碱催化法制备生物柴油的工艺条件。水酶法提取文冠果油脂的适宜条件为：Alcalase 2.4L蛋白酶用量为2ml/100g、温度55℃、pH=9、固液比1:6，在此条件下，酶水解4h，油脂提取率和蛋白水解度分别达到78.67%和9.15%。碱催化文冠果油脂制备生物柴油的最佳条件为：温度60℃、醇油摩尔比6：1、催化剂用量1.3%，在此条件酯交换反应30min，脂肪酸甲酯得率为98.81%。 建立了高温气相色谱分析油脂转酯反应样品中各种脂肪酸甲酯和脂肪酸甘油酯的方法。样品经硅烷化处理后分流进样，使用VF-5HT高温毛细柱，优化后的程序升温使各种脂肪酸甲酯和甘油酯得到良好分离，同时缩短了分析时间；十三酸甲酯和辛酸三甘油酯双内标分别对脂肪酸甲酯和脂肪酸甘油酯定量，提高了分析的准确度。本方法为油脂转酯反应制备生物柴油供了分析依据。 |
| 英文摘要 | Biodiesel is a clean alternative energy source of petroleum-based diesel because of its environment beneficial and renewable properties. But material sources and their cost used to biodiesel production restrict its development. X. sorbifolia Bunge is a good oil plant with higher oil content, which can also grow in infertile field. So it can be used as biodiesel material. The process of biodiesel production from X. sorbifolia Bunge seeds oil was first studied. The optimized conditions for oil extraction using aqueous enzymatic method and base-catalyzed transesterification were analyzed by response surface methodology. The optimal parameters for oil extraction were: enzyme quantity Alcalase 2.4 L 2ml/100g, temperature 55℃, pH 9.0, the ratio of solid to water 1:6 and enzyme incubation time 4 h. Under these conditions, the oil extraction rate and degree of protein hydrolysis reached 78.67% and 9.23% respectively. The optimal conditions for base-catalyzed biodiesel production were: temperature 60℃, molar ration of methanol to oil 6:1, amount of KOH 1.0% (w/w) and reaction time 30min. The maximum fatty acid methyl esters yield was 98.81% under above conditions. A high temperature gas chromatography method was established for analyzing fatty acid methyl esters (FAME) and mono-glycerides (MG), di-glycerides (DG) and triglycerides (TG) obtained by the transesterification of vegetable oils. Silylated sample was injected in the split mode and analyzed with a VF-5HT capillary column. Complete separation of various esters and glycerides was obtained by a programmed temperature. Methyl tridecanoate and glyceryl trioctanoate served as internal standards separately when the quantities of FAMEs and glycerides were determined .The method is very suitable for analyzing the conversion process of the oil to biodiesel. |
| 语种 | 中文 |
| 公开日期 | 2013-09-13 |
| 页码 | 85 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/1159]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | 刘苗苗. 文冠果油脂的水酶法提取及生物柴油制备[D]. 过程工程研究所. 中国科学院过程工程研究所. 2007. |
入库方式： OAI收割
来源：过程工程研究所
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