离子液体溴化1-乙基-3-甲基咪唑在青蒿素提取中的应用
文献类型：学位论文
| 作者 | 夏禹杰 |
| 学位类别 | 硕士 |
| 答辩日期 | 2008-05-29 |
| 授予单位 | 中国科学院过程工程研究所 |
| 授予地点 | 过程工程研究所 |
| 导师 | 赵兵 |
| 关键词 | 青蒿素 离子液体 溴化1-乙基-3-甲基咪唑 超声强化 大孔树脂再生 |
| 其他题名 | Application of Ionic Liquid 1-ethyl-3-methylimidazolium Bromide in the Extraction of Artemisinin |
| 学位专业 | 生物化工 |
| 中文摘要 | 本论文针对现有青蒿素提取过程中使用易燃、易爆、强挥发性有机溶剂存在的损耗高、污染环境、存在安全隐患、提取时间长、收率低等问题，研究建立了离子液体溴化1-乙基-3-甲基咪唑([emim]Br)提取青蒿素，乙酸乙酯萃取富集青蒿素和离子液体提取剂再生新技术，为开发绿色高效的青蒿素提取新技术，解决目前青蒿素规模化生产中的技术难题奠定了基础。 本论文研究建立了[emim]Br制备工艺，得到了[emim]Br制备较佳工艺条件为：磁力搅拌转速400 r/min，滴加溴乙烷结束后继续搅拌90 min。 建立了反相高效液相色谱(RP-HPLC)直接检测[emim]Br-水体系中青蒿素含量的方法，并将[emim]Br-水体系的RP-HPLC检测青蒿素的方法应用于挥发性溶剂体系，实现了用同一种检测方法检测挥发性和非挥发性溶剂中青蒿素含量。对该方法从线性关系、精密度、重复性、稳定性、最低检测限、最低定量限、回收率等方面进行评价。 通过正交实验以及单因素实验确定了超声强化[emim]Br-水体系提取青蒿素的较佳条件为：青蒿素粉末粒度40目，液固比50:1，提取时间30 min，提取温度20℃，提取功率600 W，占空比1.6s/0.4s。并将新工艺下青蒿素提取率与石油醚索氏提取青蒿素提取率进行了比较，新工艺提取率达到索氏提取的97.33%。 优化了乙酸乙酯萃取[emim]Br-水体系中青蒿素的工艺。经3次萃取可以使青蒿素萃取率达到93.38%。 本文还研究了[emim]Br-水体系提取剂的大孔树脂再生方法和循环使用。通过筛选，ADS-17具有较好的再生效果。 |
| 英文摘要 | In order to solve the problems such as high energy consumption, pollution, hidden trouble of safety, long operation time and low yield causing by using flammable and explosive volatile organic solvents in present artemisinin production, this thesis established a novel technique for the extraction of artemisinin using ionic liquid 1-ethyl-3 -methylimidazolium Bromide([emim]Br). The technique of using [emim]Br to extract artemisinin from Artemisia annua L., using ethyl acetate to extract and concentrate artemisinin and regeneration of ionic liquid laid a foundation for green and efficient large scale production of artemisinin. A process of [emim]Br preparation in laboratory was established and the optimal conditions were: rotate speed of magnetic stir 400 r/min, 90 min of continuing stir after ethy bromide was added. A reverse-phase high performance liquid chromatography (RP-HPLC) method was developed to directly determine the artemisinin content in [emim]Br-water system. Applying the RP-HPLC method to volatile solvent system and the aim of determination of artemisinin in different volatile solvents using the same method was achieved. The method was evaluated in linearity, precision, reproducibility, stability, LOD, LOQ and recovery. Through orthogonal and single factor experiments, the optimal process of artemisinin extraction using [emim]Br-water system enhanced with ultrasonic wave was determined: artemisinin granularity 40 mesh, liquid-to-solid ratio 50:1, extraction time 30 min, extraction temperature 20℃, extraction power 600 W, duty-ratio 1.6s/0.4s. Compared the results of the new process with soxhlet extraction using petroleum ether, the extraction rate of the former method was 97.33% of the latter. The process of artemisinin extraction using ethyl acetate from [emim]Br-water extractant was optimized. Extracted 3 times, the accumulative artemisinin extraction rate was 93.38%. The regeneration of [emim]Br-water extractant by different macroporous resin and its circulation use were studied. Through screening, results showed that regeneration effect of ADS-17 was better. |
| 语种 | 中文 |
| 公开日期 | 2013-09-13 |
| 页码 | 99 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/1228]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | 夏禹杰. 离子液体溴化1-乙基-3-甲基咪唑在青蒿素提取中的应用[D]. 过程工程研究所. 中国科学院过程工程研究所. 2008. |
入库方式： OAI收割
来源：过程工程研究所
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