青蒿酸高效提取及与青蒿素同步纯化
文献类型：学位论文
| 作者 | 孙景灿 |
| 学位类别 | 硕士 |
| 答辩日期 | 2009-05-31 |
| 授予单位 | 中国科学院过程工程研究所 |
| 授予地点 | 过程工程研究所 |
| 导师 | 赵兵 |
| 关键词 | 青蒿酸 超声强化提取 响应面分析法 青蒿素 纯化 |
| 其他题名 | High Efficient Extraction of Artemisinic Acid and Synchronized Purification with Artemisinin |
| 学位专业 | 生物化工 |
| 中文摘要 | 针对低青蒿素含量青蒿资源利用及青蒿酸制备中存在的问题，本论文所建立的超声强化提取青蒿酸的工艺克服了广泛采用的溶剂浸提工艺提取时间长、溶剂消耗大等缺陷，并采用液-液萃取和柱层析实现青蒿酸和青蒿素同步富集和纯化。本文主要研究结果如下： 建立了反相高效液相色谱检测青蒿酸含量的方法，该方法精密度高、稳定性和重现性好，适合用于青蒿酸的快速定量检测。 通过正交实验和单因素实验得到优化的提取条件为：粒度80目，乙醇浓度为75%，液固比30:1，提取温度40 ℃，占空比1.6 s/0.4 s，提取时间40 min，青蒿酸提取率为1.040 mg/g。 采用响应面分析法建立的青蒿酸提取预测模型相关性高（R2=0.975），具有较好的预测力。在预测最优条件下青蒿酸提取率实验结果为1.083 mg/g，与预测值1.110 mg/g很好吻合，比正交实验优化的提取条件下的提取率提高4.20%。。 通过液-液萃取实现了青蒿酸和青蒿素的同步富集。在相比（待萃液体积/正己烷体积）为5/2，萃取3次，每次20 min的条件下，青蒿酸和青蒿素萃取率分别达到96.4%和97.7%。硅胶柱层析纯化青蒿酸和青蒿素的最优条件为：以石油醚（60-90 ℃）-乙酸乙酯为（90:10）为洗脱溶剂，45 g硅胶，上样量为0.622 g，流速为1.2 mL/min。该条件下青蒿酸和青蒿素回收率分别为95.2 %、98.8%，纯度分别为17.3%、74.0%。通过重结晶得到了纯度为93.4% 的青蒿素，回收率为87.0%。 |
| 英文摘要 | In the light of the resource utilization of Artemisia annua L. with low content of artemisinin and the problems in the process of producing artemisinic acid, the ultrasonic enhanced extraction technique established in this thesis overcame the deficiency of traditional extraction methods with long extraction time and large solvent consumption. Through liquid to liquid extraction and column chromatography, artemisinic acid and artemisinin were enriched and purified synchronously. The main experimental results were as follows: A detection method of artemisinic acid by using reversed-phase high performance liquid chromatography was developed. Experimental results showed that this method had good stability, high precision and strong reproducibility. It was simple and accurate for detecting artemisinic acid in Artemisia annua L.. The optimized extraction process of artemisinic acid enhanced by ultrasonic wave was determined through orthogonal and single factor experiments: particle size of materials was 80 mesh, concentration of ethanol was 75%, extraction temperature was 40 ℃, duty ratio was 1.6 s/0.4 s, and extraction time was 40 min. Under these conditions, the extraction rate of artemisinic acid reached 1.040 mg/g. A mathematic model for the extraction of artemisinic acid was built by RSM (Response Surface Methodology). The model had higher precision (R2=0.975) and better prediction ability. Under predictively optimal conditions, the extraction rate of artemisinic acid was 1.083 mg/g which was close to the predicted value of 1.110 mg/g. It increased by 4.20% compared to the result under optimized conditions of orthogonal experiments. Liquid-liquid extraction results showed that artemisinic acid and artemisinin were enriched simultaneously. The extraction rate of artemisinic acid and artemisinin reached 96.4% and 97.7% respectively under the following conditions: phase ratio was 5/2(extract/n-hexane), three time extraction, each time for 20 min. The optimal conditions of silica gel column chromatography were determined: the eluent system was petroleum ether (60-90 ℃)-ethyl acetate (90:10), the weight of silica gel (100-200 mesh) was 45 g, sample load was 0.622 g, and flowrate was 1.2 mL/min. Under these conditions, the experimental results showed that the recoveries of artemisinic acid and artemisinin were 95.2% and 98.8% respectively, the purities of artemsinin and artemisinic acid were 17.3% and 74.0% respectively. After recrystallization, the purity and recovery rate of artemisnin reached 93.4% and 87.0% separately. |
| 语种 | 中文 |
| 公开日期 | 2013-09-16 |
| 页码 | 88 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/1293]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | 孙景灿. 青蒿酸高效提取及与青蒿素同步纯化[D]. 过程工程研究所. 中国科学院过程工程研究所. 2009. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。