In this article, a time-modulated transparent nonlinear active metasurface loaded with varactor diodes was proposed to realize spatial electromagnetic (EM) wave frequency mixing. The nonlinear transmission characteristic of the active metasurface was designed and measured under time-modulated biasing signals. The transmission phase can be continuously controlled across a full 360 range at 5 GHz when the bias voltage of the varactor diodes changes from 0 V to 25.5 V, while the transmission amplitude is between −2.1 dB to −2.7 dB. By applying the bias voltage in time-modulated sequences, frequency mixing can be achieved. Due to the nonlinearity of the transmission amplitude and transmission phase of the metasurface versus a time-modulated bias voltage, harmonics of the fundamental mode were observed using an upper triangle bias voltage. Furthermore, with a carefully designed bias voltage sequence, unwanted higher order harmonics were suppressed. The proposed theoretical results are validated with the measured results. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.