铁对赤潮异弯藻生长和生理的影响
文献类型：学位论文
| 作者 | 李东侠 |
| 学位类别 | 博士 |
| 答辩日期 | 2002 |
| 授予单位 | 中国科学院过程工程研究所 |
| 授予地点 | 中国科学院过程工程研究所 |
| 导师 | 欧阳藩 |
| 关键词 | 赤潮异弯藻 铁浓度 生长速率 光合作用 细胞密度 |
| 其他题名 | Effects of iron availability on growth and physiology of marine red tide alga Heterosigma akashiwo |
| 学位专业 | 生物化工 |
| 中文摘要 | 该文考察了不同铁浓度对赤潮异弯藻生长和生理的影响.结果发现:当铁浓度小于10nmol/L时,最大藻细胞密度与比生长速率均受到明显限制.当铁浓度大于100nmol/L时,最大细胞密度与比生长速率不再受铁限制,但其光合作用活性有所改变.在铁限制下细胞的光饱和光合作用速率P<,m>,暗呼吸速率R<,d>,表观光合作用效率α与铁丰富细胞相比都有所减小,而光补偿点I<,c>及饱和光强I<,k>增大.从活体吸收光谱可见,赤潮异弯藻在叶绿素c的靠近红光区的吸收峰处,由铁丰富条件下的632nm向蓝漂移了2nm.铁限制下的细胞活体吸收光谱在480nm左右胡萝卜素的吸收峰处多出了一个吸收峰,这是细胞内岩藻黄素的相对含量在铁限制下增大的结果,说明岩藻黄素蛋白复合物的相对含量的相应提高.与铁丰富条件(10μmol/LFe)相比,铁限制下的细胞在685nm荧光得率升高,补加铁后荧光明显降低. |
| 英文摘要 | The effects of iron on the growth and physiology of Heterosigma akashiwo were studied systematically and comprehensively. Cell final yields and the growth rate were limited when iron concentration was below 10 nmol/L and unlimited when the concentration was higher then 100 nmol/L. By comparison with the results under Fe-replete conditions, chlorophyll a-specific and cell-specific light saturated net photosynthetic capacity (Pmchl ° and Pmce")5 dark respiration rate (R/'1'0 and R/6") and apparent photosynthetic efficiency (achla and ace") decreased proportionately, whereas the cells became light saturated at higher irradiance under Fe stress. In vivo absoiption spectra of Fe-limited cells showed a chlorophyll absorption peak at 630 nm, 2 ran blue-shifted from the normal position of 632 nm. Low-temperature fluorescence emission spectra of the cells have one prominent chlorophyll emission peak at 685 nm. The Fe-limited cells showed an increase in fluorescence yield from 685 nm band compared to those grown under iron replete conditions. Low-temperature fluorescence excitation spectra and room-temperature fluorescence spectra indicated an inefficient excitation energy transfer in iron-limited cells. Total protein decreased in Fe-limited cell, while ca. 17 and 55 kDa soluble proteins were specifically induced in response to Fe limitation. Light limitation increased the growth rate repress from Fe limitation. Under low light condition, the growth rate and final cell yield decreased on the same N source and Fe concentrations. Under the same light condition, distinct differences in cellular NR and NiR activities on nitrate were observed in Fe replete and Fe limited cultures. On the other hand, since cells grown on ammonium salt contain negligible nitrate reductase activity, cells grown on ammonium salt no longer needed nitrate reductase. C: N ratios suggested that though NR and NiR activities decreased under Fe limitation, the effect of Fe on NH/ and NO3" did not specifically involve in the nitrate assimilatory pathway. |
| 语种 | 中文 |
| 公开日期 | 2013-09-16 |
| 页码 | 134 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/1350]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | 李东侠. 铁对赤潮异弯藻生长和生理的影响[D]. 中国科学院过程工程研究所. 中国科学院过程工程研究所. 2002. |
入库方式： OAI收割
来源：过程工程研究所
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