白蛋白纳米球药物载体的制备及其体外释药特性的研究
文献类型：学位论文
| 作者 | 王恺 |
| 学位类别 | 硕士 |
| 答辩日期 | 2003 |
| 授予单位 | 中国科学院过程工程研究 |
| 授予地点 | 中国科学院过程工程研究 |
| 导师 | 马光辉 |
| 关键词 | 白蛋白纳米球 药物载体 体外释药 丝裂霉素 |
| 其他题名 | Preparation and in vitro Release Characteristics of Albumin Nanospheres as Drug Carrier |
| 学位专业 | 化学工艺 |
| 中文摘要 | 该文以超声乳化一化学交联的方法制备了负载丝裂霉素的白蛋白纳米球,具体制备方法如下:含有丝裂霉素、氯化钠和白蛋白的水相经超声乳化,在油相中形成纳米乳滴,然后加入戊二醛使纳米乳滴发生交联形成纳米球,再以离心收集,用有机试剂洗涤数次,真空干燥得到成品.该文对白蛋白纳米球载体的制备体系和制备条件进行了优化,确定了最佳制备体系,并系统考察了制备条件对白蛋白纳米球性质及其释药特性的影响. |
| 英文摘要 | Mitomycin-loaded albumin nanospheres were prepared by ultrasonic emulsification-crosslinking method. The preparative process is as follows: after the albumin aqueous phase containing mitomycin and NaCl was emulsified into the oil phase by a ultrasonifier to obtain the nanodrolets, glutaraldehyde was added to crosslink the nanodroplets, the formed nanospheres were then separated by centrifugation and washed by organic solvents, finally they were dried under vacuum. The preparative system and conditions were optimized, and the influences of the preparative conditions on the properties and release characteristics of albumin nanospheres were investigated systematically. First, several organic solvents were tested in order to determine the feasible oil phase. It was clarified that the albumin particles can be formed only when cyclohexane or a mixture of n-hexane and dichloromethane was used as the oil phase. Because the particle yield was relatively higher and the albumin microspheres were smaller when using cyclohexane as the oil phase, the cyclohexane was used as the oil phase in this study. Then the optimum emulsifier - Arlacel 83 was determined by comparing the stability of the emulsions prepared with various emulsifiers. Afterward, the optimum condition for the preparation of albumin nanospheres was obtained: the concentration of albumin in normal saline was 20%(w/v), the volume ratio of aqueous phase to oil phase was 1:50, glutaraldehyde was used in the form of toluene solution, the power of ultrasonifier was 300W, the duration of ultrasonication was lOmin. The influence of the amount of crosslinker - glutataldehyde on the properties of the albumin nanospheres was systematically investigated. It was found that the yield and drug content of nanospheres and loading efficiency of the drug increased, while the diameter and swelling degree of nanospheres and the release rate of drug in vitro decreased with the increase of gluataraldehyde amount. The average diameters of the albumin nanospheres prepared under optimized condition were between 60 and 100 nm, the drug content and the loading efficiency attained to 1.66% and 90.15%, respectively. The drug release profile in vitro can be divided into two phases, both of which complied with Higuchi equation. The presence of trypsin increased the release rate of drug dramatically. And,.the more the amount of glutaraldehyde used, the slower the drug release from the prepared albumin nanospheres in the presence of trypsin. However, the cumulative drug release amount in the presence of trypsin was all close to 100% eventually. Finally, the influence of the addition mode of glutaraldehyde was investigated, little difference was found on properties of nanospheres prepared with continuous and batch addition modes except that the latter showed a slightly higher drug content. This study provided an important knowledge that albumin nanopsheres with the size lower than 100 nm and high loading efficiency can be prepared by ultrasonification -crosslinking technique. It can be expected that the drug carriers with long circulating time and good targeting can be developed by the surface modification of the nanospheres. |
| 语种 | 中文 |
| 公开日期 | 2013-09-16 |
| 页码 | 69 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/1361]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | 王恺. 白蛋白纳米球药物载体的制备及其体外释药特性的研究[D]. 中国科学院过程工程研究. 中国科学院过程工程研究. 2003. |
入库方式： OAI收割
来源：过程工程研究所
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