魔芋寡糖及其铬、钒络合物生物活性研究
文献类型：学位论文
| 作者 | 钱风云 |
| 学位类别 | 硕士 |
| 答辩日期 | 2003 |
| 授予单位 | 中国科学院过程工程研究所 |
| 授予地点 | 中国科学院过程工程研究所 |
| 导师 | 傅德贤 |
| 关键词 | 魔芋寡糖 铬络合物 钒络合物 生物活性 |
| 其他题名 | Biological Activities of Konjac Oligosaccharide and its Chromium and Vanadium Complex |
| 学位专业 | 生物化工 |
| 中文摘要 | 魔芋是我国西部山区资源非常丰富的一种药用植物,其酶解产物魔芋寡糖(KOS)具有调节血糖、调节肠道功能、提高免疫能力、提高超氧化物歧化酶活性的作用.铬离子是葡萄糖耐量因子的必需成分,钒具有类胰岛素效应.该文合成了魔芋葡甘露寡糖铬及其钒络合物(KOSCr、KOSV),并分别从对胰岛一氧化氮自由基(NO)含量的影响、对体外非酶促蛋白糖基化的影响以及对α-葡萄糖苷酶活性的影响三方面研究了魔芋寡糖及其铬、钒络合物在糖尿病模型中的应用.应用链脲佐菌素(STZ)致胰岛NO自由基损伤模型,发现KOS可降低NO含量约17%,KOSV未与KOS表现出明显差别.KOSCr铬含量在2.842%-3.871%之间时比KOS的效果显著,其中铬含量为3.519%时效果最显著,从而起到保护胰岛免受自由基损伤的作用.在体外牛血清白蛋白(BSA)非酶促糖基化反应模型中,KOS表现出一定的抑制作用,并呈现出浓度依赖性,在反应23天时,1mg/mlKOS组的棕色产物生成量只有糖化系统对照组的62%.KOSCr和KOSV的抑制效果与KOS相似,可见络合物的抑制作用主要是由其寡糖部分决定的,而与铬、钒离子的加入没太大关系.在α-葡萄糖苷酶活性实验中,KOSV组表现出明显的抑制酶活作用,也有良好的剂量依赖关系:10<'0>,10<'-1>,10<'-2>,10<'-3>,10<'-4>,组中酶活分别为对照组的27.8%、46.8%、57.8%、65.0%和69.2%,IC<,50>值为0.07mg/ml.最后通过实验确定了KOSV具有温度稳定性,pH稳定性,对该酶有较高亲和力,并且是一种非竞争性抑制剂.魔芋寡糖及其铬、钒络合物在以上三方面所表现出的生物活性对深入研究其在糖尿病防治中的应用具有一定的指导意义. |
| 英文摘要 | Konjac is a kind of pharmaceutically useful plant distributed abundantly in the western part of China and its enzymatic hydrolyzate is called Konjac oligosaccharide (KOS). KOS takes effects on regulating blood glucose, adjusting intestinal function, improving immunity and enhancing SOD activity. Chromium (Cr) is a necessary component of the glucose tolerance factor (GTF), while vanadium (V) is the mimics of insulin. KOS chromium complex (KOSCr) and vanadium complex (KOSV) were synthesized and their applications in diabetic models were investigated from three aspects: the effect on nitric oxide (NO) content in islets, the effect on non-enzymatic protein glycation in vitro and the effect on a-glucosidase enzymatic activity. The complexes were synthesized by adding CrCb^BkO and Na3VO4-12H2O into KOS (the enzymatic product of Konjac). The solubility, chromium content, vanadium content of each product were examined for later biological activity assay. Based on the STZ-induced diabetic islet impairment model by NO free radical, KOS and KOSV reduced the content of NO by 17%. KOSCr showed clear effect when Cr concent varied in the range of 2.842%~3.871% and the maximal effect was observed at 3.519%. The results show that KOSCr protects islets from the damage of NO free radical. KOS exhibited an inhibitory activity on the non-enzymatic glycation of bovine serum albumin (BSA) in vitro with concentration dependence. The inhibitory rate of lmg/ml KOS was 62% at the 23rd day. Because the effects of KOSCr and KOSV had no difference to KOS, it is concluded that the inhibitory effect is mainly determined by the oligosaccharide part in the complex, instead of Cr or V. KOSV effectively inhibited a-glucosidase enzymatic activity by a concentration dependent relationship. The enzyme inhibitory rate in the rank of 10°, 10"1,10"2,10"3 and 104 were 27.8%, 46.8%, 57.8%, 65.0% and 69.2%, respectively, of that of control and IC50 value was 0.07mg/ml. KOSV was proved to be steady in a wide range of temperature, pH and had high binding ability with a-glucosidase. It was a kind of non-competitive inhibitor. The biological activities shown by KOS, KOSCr and KOSV are instructive to their further research and application in diabetes treatment. |
| 语种 | 中文 |
| 公开日期 | 2013-09-16 |
| 页码 | 71 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/1368]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | 钱风云. 魔芋寡糖及其铬、钒络合物生物活性研究[D]. 中国科学院过程工程研究所. 中国科学院过程工程研究所. 2003. |
入库方式： OAI收割
来源：过程工程研究所
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