新型固态发酵真菌杀虫剂
文献类型：学位论文
| 作者 | 刘健 |
| 学位类别 | 硕士 |
| 答辩日期 | 2003 |
| 授予单位 | 中国科学院过程工程研究所 |
| 授予地点 | 中国科学院过程工程研究所 |
| 导师 | 陈洪章 |
| 关键词 | 真菌杀虫剂 固态发酵 压力脉动 过程控制 微机在线监控系统 |
| 其他题名 | Novel Solid-State Fermentation of Fungal Pesticide |
| 学位专业 | 生物化工 |
| 中文摘要 | 该论文针对真菌杀虫剂现有生产工艺中存在的问题,以其代表菌白僵菌、绿僵菌为实验对象,从菌种筛选入手,重点研究新型固态发酵工艺——真菌杀虫剂的压力脉动固态发酵,以期为进一步的工业放大提供依据.采用常规固态发酵方式系统研究了上述优选菌株固态发酵的条件需求,对影响菌丝生长特别是产孢的各种因素进行了系统探讨,给出了优化的工艺条件.在此基础上,重点进行了白僵菌、绿僵菌的压力脉动固态发酵研究,从多方面考察了压力对发酵的影响,确定了低压0.0MPa,高压瞬时的脉动策略;结合压力脉动反应器的特点,对压力脉动固态发酵过程参数进行了系统优化与控制;在该实验系统优化的压力脉动固态发酵工艺条件下,白僵菌、绿僵菌产孢量分别达325亿/克和165亿/克,比常规静止固态发酵分别提高60%和50%,发酵周期均缩短一天.分析压力脉动固态发酵过程中O<,2>和CO<,2>浓度的变化规律,针对具有白僵菌、绿僵菌类发酵特性的微生物提出了一种新型的压力脉动操作方式.初步建立了压力脉动固态发酵过程的微机在线监控系统,并提出了真菌杀虫剂压力脉动固态发酵过程的微机在线优化控制研究设想. |
| 英文摘要 | In this thesis, air pressure pulsation solid-state fermentation (SSF), a novel SSF, was applied to produce fungal pesticide using screened strains from Beauveria and Metarhizium. This work was to make out some problems in the fungal pesticide production process and to set groundwork for scale-up of the industrial production. Firstly, the first-rate strains of Beauveria bassiana and Metarhiziuml*, which produced high virulence and had other good properties, were selected by screening from fungi in infected worms collected in forests and stains used in industrial production and by ultraviolet radiation. Secondly, the fermentation conditions were optimized by general SSF using the above selected strains to improve the productivity of spores. On the basis of the above research, air pulsation SSF was adopted to produce fungal pesticide using Beauveria bassiana and Metarhiziuml#. The effects of air pressure variation were investigated and a new air pressure pulsation method was brought forward. According to the new method, the air pressure was discharged promptly to the low air pressure of 0.0 Mpa as the air pressure got to a given peak . The fermentation conditions were optimized and controlled during the air pressure pulsation SSF on the basis of the properties of the air pressure bioreactor. At the optimized conditions, the cultivation period was 6 days, one day shorter than that of general SSF. The spore productivity of Beauveria bassiana got to 32.5 billion/g, 60% higher than that of general SSF and the spore productivity of Metarhiziuml* got to 16.5 billion/g, 50% higher than that of general SSF. Also the O2 and CO2 concentration variations were investigated during air pressure pulsation SSF. A modified pulsation was introduced according to the characters of these strains, such as Beauveria bassiana and Metarhizium^'. An on-line monitoring system by computer was set up, and a new idea about on-line optimizing and monitoring of the parameters was come up with during air pressure pulsation SSF to produce fungal pesticides. |
| 语种 | 中文 |
| 公开日期 | 2013-09-16 |
| 页码 | 77 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/1369]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | 刘健. 新型固态发酵真菌杀虫剂[D]. 中国科学院过程工程研究所. 中国科学院过程工程研究所. 2003. |
入库方式： OAI收割
来源：过程工程研究所
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