裙带菜无性繁殖系的生理特性及反应器培养
文献类型：学位论文
| 作者 | 张栩 |
| 学位类别 | 博士 |
| 答辩日期 | 2003 |
| 授予单位 | 中国科学院过程工程研究所 |
| 授予地点 | 中国科学院过程工程研究所 |
| 导师 | 欧阳藩 |
| 关键词 | 裙带菜 无性繁殖系 光生物反应器 生理特性 |
| 其他题名 | Physiological Properties and Cultivation in Photobioreactor of Undariapinnatiflda clone |
| 学位专业 | 生物化工 |
| 中文摘要 | 裙带菜(Undariapinnatifida)作为海洋中的初级生产者,将成为21世纪人类食物、药物和化工原料的重要来源.裙带菜的传统养殖技术效率低、特别是良种化程度低已制约了其发展.基于裙带菜无性繁殖系的生理特性,将光生物反应器等生化工程技术用于其培养和扩增,是实现良种化、规模化和产业化的关键.该文以裙带菜无性繁殖系的雌、雄配子体和幼孢子体为实验研究对象,实验证明裙带菜具有蓝光效应,即蓝光能促进配子体的生长、发育、光合放氧和无机碳利用能力,配子体所吸收400~500nm光量子数决定其发育率的高低,超过800μmolcm<'-2>时,发育率达到100%;蓝光也明显促进了幼孢子体光合色素合成和光合作用能力.利用光生物反应器培养的裙带菜配子体,能健康生长并发育为幼孢子体,通过室内育苗和海上养殖,成为商品.上述实验结果将为裙带菜无性繁殖系育苗法的产业化提供理论依据和技术支持. |
| 英文摘要 | As a primary producer of marine creatures, Undaria pinnatiflda would be one of the important sources of food, medicines and chemical materials in the 21st century. The traditional aquiculture of U, pinnatifida has restricted its development. The biochemical engineering technology including photobioreactors utilized in the cultivation of gametophytes clone is an effective alterative to the seed-breeding and commercial aquiculture of U. pinnatifida. In this theesis, female and male gametophytes and juvenile sporophytes of U. pinnatifida were as the marterials. The photosynthetic characteristics of gametophytes were different from that of juvenile sporophytes. In fluorescence emission spectra at 77 K, gametophytes had fluorescence emission maximum at 741 run, which was seen as the characteristic of PS I, while at 705 nm for juvenile sporophytes. At 17°C, the maximal net photosynthetic rate of female gametophytes (71.16 umolO2 (mg Chi a)"1 h"1), was significantly higher than that of juvenile sporophytes (139.98 umol O2 (mg Chi a)"1 h'1). Gametophytes in a close system had low ability to utilize inorganic carbon and its pH compensation point was at 8.80 and pH 9.53 for juvenile sporophytes. Blue light accelerated the growth, development and photosynthesis of gametophytes, which were called "Blue-light respond". The fertility of gametophytes was dependent on quantum doses in 400~500 nm. The fertility was 100% as soon as quantum doses exceeded 800 umol cm'2. Furthermore, the synthesis of pigment and photosynthesis in juvenile sporophytes were strengthened by blue light. With aeration rate at 1.0 1 min'1, the final biomass of gametophytes in 2.5 L airlift reactor (0.71 mg DW ml"1) was higher than that in 2.5 L bubble column (0.59 mg DW ml"1). In the cultivation of gametophytes in the airlift photobioreactor, dissolved oxygen in culture was not overabundance to restrain growth. The capabilities of photosynthesis and respiration of gametophytes can be measured by on-line monitoring pH in the cultivation process. Higher concentration of CO2 and blue light were simultaneously used, which was beneficial to the growth and development of gametophyte clone. When CO2 concentration was 0.25%, the final biomass was 0.78 mg DW ml"1. However, the growth was restrained as the CO2 concentration was raised to 0.5%. Higher concentration of CO2 decreased the photosynthesis of gametophytes. When CO2 concentration was 0.25%, the maximal net photosynthetic rate and total carbonic anhydrase activity decreased by 14% and 49%, respectively. But the inhibition was eliminated by the irradiation of blue light, so that the final biomass reached 0.90 mg DW ml"1. Through the cultivation in the airlift photobioreactor, the gametophytes clone of U, pinnatifida could grow well and develop to juvenile sporophytes for indoor breeding. Subsequently, young sporophytes were cultivated in sea and grew to the commercial sporophytes. The research results would serve as the theory basis and technology support for the commercial aquiculture of U. pinnatifida clone. |
| 语种 | 中文 |
| 公开日期 | 2013-09-16 |
| 页码 | 168 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/1374]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | 张栩. 裙带菜无性繁殖系的生理特性及反应器培养[D]. 中国科学院过程工程研究所. 中国科学院过程工程研究所. 2003. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。