耐高渗酵母多级连续发酵生产甘油的研究
文献类型：学位论文
| 作者 | 刘永强 |
| 学位类别 | 博士 |
| 答辩日期 | 2003 |
| 授予单位 | 中国科学院过程工程研究所 |
| 授予地点 | 中国科学院过程工程研究所 |
| 导师 | 刘德华 |
| 关键词 | 多级串联连续发酵 甘油 耐高渗酵母 渗透压 稀释率 限制性底物 玉米浆 |
| 其他题名 | Study on continuous glycerol production by Candida krusei in a multistage cascade bioreactor |
| 学位专业 | 生物化工 |
| 中文摘要 | 耐高渗酵母发酵法生产甘油是生物法生产甘油的主要方法.为了提高生物法生产甘油的生产效率,降低生产成本,国内外同行对耐高渗酵母连续发酵生产甘油进行了研究.由于耐高渗酵母发酵生产甘油对发酵液渗透压有一定要求,所以单级连续发酵系统所取得的结果远低于间歇发酵.基于耐高渗酵母发酵生产甘油的机理,该文首次提出采用多级串联发酵系统进行甘油的连续生产,并对此过程中的主要影响因子即发酵过程所需维持的渗透压、稀释率、限制性底物以及富含营养成分的玉米浆等因素对连续生产甘油的影响进行了研究。 |
| 英文摘要 | Glycerol production by osmophilic yeast is the main way for microbial production of glycerol. In order to improve the efficiency of glycerol production by fermentation, continuous fermentations were studied. However, results of single continuous fermentation were not better than those of batch fermentation because the requirement of certain osmophilic pressure could not be satisfied. Based on the regulation mechanism of the osmophilic yeast under osmophilic condition, the production of glycerol by continuous fermentation with free Candida krusei in a multistage cascade bioreactor was developed. Main factors affecting continuous preparation of glycerol in the multistage system such as osmotic pressure, dilution rate, limiting substrate and com steep liquor were investigated. In order to find out the stability and the resistance to contamination of strain used in the study for long-term operation, repeated batch fermentation was conducted. Results showed that the strain had excellent stability and the fermentation process was not easy to be contaminated in the long-term operation. In twelve cycles of repeated batch fermentation, average glycerol concentration, glycerol yield, and glycerol productivity was 71 g I"1, 35.5%, and 26.0 g I"1 d"1 respectively, which were better than those of reports in references. The study of multistage cascade system proved that maintaining the certain osmophilic pressure was the key factor for successful continuous fermentation. Glycerol concentration, glycerol yield and productivity were improved with either NaCl or glucose as osmoregulator. Dilution rate is another important factor affecting continuous fermentation. When glucose concentration in feeding medium was constant, both specific glycerol productivity and glycerol productivity increased with the increase of dilution rate, but the increase degree of specific glycerol productivity was much higher than that of the productivity. In addition, carbon source was not appropriate to be limiting substrates, while nitrogen limitation benefited the improvement of glycerol yield based on the glucose consumed and phosphorus limitation favored the increase of glycerol productivity. Corn steep liquor, which was often used as phosphate source, had important effect on glycerol fermentation because of the abundant nutrition,. KH2PO4 was used as phosphate source and 1.0 g I"1 corn steep liquor as nutrition was added into the medium in this study. Results showed that addition of corn steep liquor improved the glycerol productivity greatly and fermentation process was stable. Glycerol concentration of 123.6 g I"1, glycerol yield of 37.3% and glycerol productivity of 35.6 g I"1 d"1 were obtained, respectively, when feed glucose concentration and feed corn steep liquor concentration were 370 g I"1 and 1.0 g I*1 in a five-stage cascade system. Compared with batch fermentation, glycerol yield and glycerol productivity were improved by 20.8% and 106.4% respectively while glycerol concentration was almost the same. |
| 语种 | 中文 |
| 公开日期 | 2013-09-16 |
| 页码 | 128 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/1377]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | 刘永强. 耐高渗酵母多级连续发酵生产甘油的研究[D]. 中国科学院过程工程研究所. 中国科学院过程工程研究所. 2003. |
入库方式： OAI收割
来源：过程工程研究所
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