稀土元素对雪莲细胞培养的影响与反应器技术
文献类型：学位论文
| 作者 | 王谦 |
| 学位类别 | 硕士 |
| 答辩日期 | 2001-06 |
| 授予单位 | 中国科学院研究生院 |
| 授予地点 | 北京 |
| 导师 | 王玉春 |
| 关键词 | 稀土元素 雪莲 黄酮类化合物 细胞培养 生物反应器 |
| 其他题名 | Effects of Rare Earth Elements on Saussurea medusa Cultures and Bioreactor Technology |
| 学位专业 | 植物细胞工程 |
| 中文摘要 | 雪链生长环境特异，天然生长缓慢，人工栽培困难，长期的掠夺式采挖已使雪莲成为濒危物种.应用植物细胞培养技术进行雪链细胞的培养既可满足临床上日益增长的需求，又可能保护自然资源，具有重要的现实意义.1、稀土元素对三角瓶培养的雪莲细胞的影响.该论文选择文献报道对其他植物的生长和次级代谢具有促进作用的La、Ce、Nd3种稀土元素和一种混合稀土Re，分别研究了固体培养和摇瓶液体培养时培养条件及稀土元素对水母雪莲Saussureamedusa(SM-01)细胞生长和次生代谢产物正确性的影响.2、2升倾斜筛网气升式反应器培养.2升倾斜筛网升式植物细胞培养反应器中的研究结果表明：在反应器中使用三层筛网时，细胞聚集体会逐渐附着在筛网或者支架上生长，第2层筛网上的细胞达到反应器内细胞总量的41.46%;第1层筛网上的细胞中的酮类化合物的含量最高，可以达到34.6mg/gDW. |
| 英文摘要 | Medusa Saussurea is well known as a valuable traditional Chinese medicine which grow in a special environment. Because of its difficult collection and artificial cultivation, as well as being digged illegally over a long period of time, Medusa Saussurea is becoming scarce plant source. So, the production of medicinal second metabolite in Medusa Saussurea by the culture of plant cells in a novel bioreactor has considerable significance not only in the protection of natural plant resource, but also in its academic research and commercial interest. This thesis investigated the effects of rare earth elements and inoculum concentration on the growth of Saussurea medusa(SM-01) cells and the synthesis of flavonoid. In addition, a novel airlift bioreactor with inclined meshes was designed. A study on the growth of the cells and the production of flavonoid in the bioreactor in a range of inoculum concentrations and air flow rates was carried out. 1. Effects of rare earth elements on Saussurea medusa cell cultures When Ce initial concentration was 0.05mmol/l in the solid medium with 6-BA and NAA at normal concentrations, the biomass, flavonoid content and flavonoid production were highest at the values of 420.6gFW/l-medium, 52.4mg/gDW and 1102mg/1·medium respectively. They were 169.2%, 120.2% and 203.3% of those without rare earth elements. In 100ml Erlenmeyer flask with 25ml liquid medium, 80gFW/l-medium inoculum concentration resulted in the highest specific growth rate of 0.252day"', and 120gFW/l-medium inoculum concentration resulted in the highest flavonoid content of 43.6mg/gDW. When inoculum cells aged 12 days, the highest biomass of 418.9g/l-medium was. obtained. The cells aged 21 days resulted in the highest flavonoid content of 43.1 mg/gDW, and the cells aged 15 days resulted in a maximal flavonoid production of 678 mg/l-medium. The experimental results obtained on a rotary shaker at llOrpm showed that cell aggregates sized 4mm to 6mm reached 28.8% of the total cell weight. The size range of cell aggregates with the highest average flavonoid content was from 8mm to 10mm which occupied 16.7%, the average content reached 49.6 mg/gDW. When the initial concentration of Ce was 0.025mmol/l in liquid medium with 6-BA and NAA at normal concentrations, the biomass, flavonoid content and flavonoid production had maximal values, they were 443.5gFW/1·mediumJ 53.1 mg/gDW and 942mg/1·medium, which were 134.4%, 124.1% and 166.7% of those without rare earth elements respectively. 2. Cultures in 2L airlift bioreactor with inclined meshes When three meshes were arranged in the bioreactor, the cell aggregates attached on the meshes and grew well. The cultivated results showed that the biomass on the second mesh reached 41.46% of the total weight, and the cell aggregates on the first mesh had a maximal flavonoid content of 34.6 mg/gDW. The results of inoculation concentration effects showed that when the inoculation concentration was 16.227gFW/l-medium, the specific growth rate and flavonoid content reached 0.128day~(-1) and 33.7mg/gDW respectively. The source of inoculum also affected the cell growth and flavonoid formation. The inoculum used came from solid medium, the specific growth rate reached 0.128day~(-1) which was lower than that(0.138 day~(-1)) when inoculum used came from liquid medium. However, the flavonoid content reached 33.7 mg/gDW, which was higher than the latter(31.2 mg/gDW). When two meshes were fixed in the bioreactor, the cell aggregates can circulated. In this situation, air flow rate was a key factor affected the cell cultivation. When air flow rate was 0.05m3/h, a maximal specific growth rate of 0.097day~(-1) was obtained, at the same time, flavonoid content and flavonoid production were also highest, they were 30.4 mg/gDW and 98.6mg/limedium respectively. Under the same culture conditions, the specific growth rate and flavonoid content of Saussurea medusa (SM-01) got from mesh-attached state reached 0.128day~(-1) and 33.7mg/gDW, which were all higher than those got from circulated culture state. |
| 公开日期 | 2013-09-26 |
| 页码 | 117 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/1899]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | 王谦. 稀土元素对雪莲细胞培养的影响与反应器技术[D]. 北京. 中国科学院研究生院. 2001. |
入库方式： OAI收割
来源：过程工程研究所
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