色谱填料信息系统——制备用填料数据库及离子交换填料评估系统
文献类型：学位论文
| 作者 | 李伟 |
| 学位类别 | 博士 |
| 答辩日期 | 2000-05 |
| 授予单位 | 中国科学院研究生院 |
| 导师 | 欧阳藩 |
| 关键词 | 蛋白质 液相色谱 填料 数据库 综合评估 信息系统 |
| 其他题名 | Chromatographic Media Information System--Preparative Media Databases and Ion Exchanger Evaluation System |
| 学位专业 | 化学工艺 |
| 中文摘要 | 液相色谱是现代生物主品分离的主要手段。对一个企业而言，能在研究开发阶段快速而系统的选择到合适的填料，意味着可能在比较短的时间里找到合适的工艺，以较低成本的产品尽早进入市场，在竞争中取得优势。目前对研究开发人员来说面临几项困难：一是手头数据资料不全；二是缺乏一个系统比较评估的体系，故即使掌握了较多厂家的填料产品数据了很省能充分利用；三是实际分离问题本身较为复杂，对填料有特殊要求，这更增加了问题的难度，使之难以在短时间内得到满意解决。本文针对上述问题，首先构建了制备性离子交换填料数据子库，库中收集了106种离子交换填料的相关数据。包括填料共性，填料个发两张表，前者集中了同类填料的共同性质，如颗粒大小及其分布，孔径，机械强度及在常见化学试剂包括有机溶剂、变性剂、氧化剂中的稳定性；后者集中了随填料不同而变化的各方面属性，如填料的离子容量及键合相密度，填料吸附量及在酸碱性环境中的稳定性，填料在不同溶剂环境中的涨缩性。除离子交换填料数据子库外，还有两个与填料使用相关的数据子库，即文献数据子库与商业住处子库。前者包括核心文献子库和应用文献子库：其中核心文献子库由文献表和文献－填料表组成，收录的是厂家镇对斯填料产品所发表的文献，对了解填料产品至关重要；应用文献子库由应用文献表构成，包括了重要填料商品的应用文献，重点针对填料的具体应用。目前收录核心文献42篇，应用文献2000余篇，两种文献均含题目、关键词及摘要等相关数据。商业信息子库厂家和代理两张表，汇集了与产品定货相关的信息。可加速采购决策与定货。上述数据库的浏览与查询也在本章做了做了讨论。在全面分析文献的基础上，针对分离实际问题，确立了制备性离子交换填料的评估体系。生产中最常追求的是产率最大化，本文以些为目标，将影响产率的因素逐项分解，与离子交换填料相应的指标对应。同时考虑到实际操作的可行性，确立了评估预选指标集。通过统计学方法对文献报道的30种阴离子交换填料数据进行了产成分分析，并且统计分析了38篇生化分离用离子交换填料的综合评估文献，表明离子交换填料综合评估中指标选取最常见的是吸附容量、流速和回收率，由此证明本文评估系统指标选取的合理性。在上述工作的基础上，将离子交换填料的筛选与评估体系具体化，主要包括两方面的工作：一是人实用角度出发，设计制作了离子交换填料的筛选界面，通过些界面可以很方便的根据需要，将符合使用者要求的填料人数据库中选择出来。二是设计制作了两评估系统：一是完全基于填料库中已有的数据，在条件限定（筛选）的基础上，进行粗略的评估：别一种是利用实验测定部分数据，进行较为准确的评估，最后利用报道的数据，比较了两种评估系统的特点。前面两方面的工作均以离子交换填料为讲座对象，构建了填料数据库及其的评估系统。论文的第三部分收录了疏水型 填料及凝胶过滤填料的数据并讨论了相应的检索与查询。两类数据库均由两种表构成，即个性与共性表。疏水／反相填料个性表包含了填料键合相种类与键合密度，吸附量，酸碱稳定性；共性表包含了填料颗粒大小及分布，孔径大小及排阻上限，最大耐压及流速，在氧化剂，有机溶剂，变性剂及表面活性剂 中的稳定性等。凝胶过滤填料个性表包含了填料成分，排阻上限及分离范围，耐压上限；共性表包含了填料酸碱稳定性，及在常用试剂中的稳定性等。厂家与代理等信息可人离子交换填料商品及住处库中获取。目前收录17种疏水填料及76种凝胶过滤填料的相关数据。这样，与前面的工作一起构成了比较完整的填料数据库信息系统。本文中的数据库管理系统采用Microsoft Access 2000，编程采用Visual Basic for Application (VBA),目前文件大小为26兆字节。 |
| 英文摘要 | Liquid chromatography is one of the most frequently used methods in modern bioseparation. To choose the right packing within a limited period of time guarantees a manufacture to enter the market earlier at a possibly lower cost than its counterparts so that the company can succeed in the competition. There are several obstacles that the R&D researchers may encounter in media selection. The first is the lack of data on commercial matrices and their producers. The second is that there is not any comprehensive evaluation system available for preparative liquid chromatographic media, consequently the limited amount of data at hand cannot be used adequately. The third is the complexities in practical separation often request a flexible rather than stable evaluation on the matrices. All these puzzle the researchers in process design in modern biotech industry. Targeting on those problems, firstly we have constructed a subdatabase on preparative ion exchanger, which is the most commonly used packing in protein separation. The subdatabase is made up of two tables, "the common" and "the specific". "The common" table consists of the fields on qualities commonly for a class of media such as particle diameter and distribution, pore size, maximum flow rate and the chemical stability in oxidants, detergents, organic solvents and denaturants. "The specific" table consists of fields on qualities varying with different media such as small ion capacity, protein binding capacity and the stability in alkaline/acid solutions, the swelling and shrinking with variations in composition and concentration of commonly used solution like salts, organic solvents, during chromatographic process. There are 106 ion exchangers' data been collected at present. In addition, two other subdatabases, the literature and the commercial information subdatabases have also been built. The literature subdatabase is made up of two subdatabases: the key reference and application reference subdatabase. The former collects 42 references given by the manufactures on their media products and is vital for media selection in practice. The latter collects over 2000 application references of the commonly used media. The commercial information subdatabase is made up of two tables: "manufacture", "agent". It contains information on producers and product prices and can help the users in inquiring and making order. The data query and management is also discussed. The second part of this dissertation is the comprehensive evaluation of preparative ion exchangers. Productivity is taken as the target, which is the same case as in practice. First, an evaluation parameter set is put forward based on an extensive analysis of literature. The parameters chosen are all closely related to productivity and can also be easily acquired in practice. Factor analysis on reported data of 30 anion exchangers indicates that factors contributed much to the total variance of the separation correspond to the three factors of productivity. Also, statistics from 38 references on ion exchanger characterization are made which shows that parameters appear most frequently in these references are exactly the same as evaluation parameters being chosen in this article. These results support the soundness of the evaluation parameter set. Second, the comprehensive evaluation system is realized by Visual Basic programming. The system consists of three parts. The first is the selection interface which can select media according to the rigid requirements in bioseparation, mostly on the stability of matrix. The second is a general evaluation interface which can be used for reported data from the database or elsewhere. The third is a relatively accurate systematic evaluation interface which utilizes the experimental data acquired by the user. Both interfaces can input weights for different evaluation parameters to accommodate the practical requirements to select satisfying media. The last part of this dissertation is the database construction of hydrophobic/reverse phase and gel filtration media. The query and management of the above subdatabases are presented. The three subdatabases on media and the evaluation system along with the literature and commercial subdatabases form a preparative matrix information system to provide a systematic solution for media selection in modern bioseparation. The database management system is Microsoft Access 2000. The file is 26M bytes at present. |
| 语种 | 中文 |
| 公开日期 | 2013-09-26 |
| 页码 | 177 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/1932]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | 李伟. 色谱填料信息系统——制备用填料数据库及离子交换填料评估系统[D]. 中国科学院研究生院. 2000. |
入库方式： OAI收割
来源：过程工程研究所
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