蓝藻在光生物反应器中的光自养培养
文献类型：学位论文
| 作者 | 康瑞娟 |
| 学位类别 | 硕士 |
| 答辩日期 | 1999-08-30 |
| 授予单位 | 中国科学院研究生院 |
| 导师 | 蔡昭铃 ; 施定基 |
| 关键词 | 微藻 鱼腥藻7120 聚球藻7002 光生物反应器 培养 光衰减 |
| 中文摘要 | 微藻的高密度培养是微藻生物资源利用的关键，高效的封闭式光生物反应器是实现藻细胞高密度培养的首要条件和微藻生物技术的发展趋势。本文首先对两种藻细胞培养液中光的衰减规律进行了研究，得到了不同藻细胞浓度下光强随光程距离衰减的数学关联式，能够较好地描述光强在藻液中的变化情况。发现并证明了光的衰减不仅与细胞密度和光程距离有关，而且取决于藻细胞的大小、形态等生物学特性。在文献调研和实验的基础上，设计构建了一套15升内外光源相结合的内环流气升式光生物反应器系统，对反应器的主要性能参数进行了测定，表明该反应器适合于藻细胞高密度培养得要求。以两种不同形态的微藻一丝状体淡水蓝藻鱼腥7120和单细胞海洋蓝藻为对象，进行了蓝藻光自养培养实验，考察了环境因子和反应器操作条件对藻细胞生长的影响。确定了鱼腥藻7120和聚球藻7002优化的培养工艺和操作条件，达到了较高的培养密度，并为进一步培养转基因微藻奠定了基础。 |
| 英文摘要 | Microalgae are very important bioresource, its high cell density cultivation in an enclosed photobioreactor is the key factor to increase its production. In this paper, the light intensity attenuations in the culture systems of two kinds of microalgae were studied first. The light attenuation mathematical models established through regressive analysis of experimental datas, which could describe the changes of light intensity properly in the culture systems. The relationship between light attenuation and size of the microalgae cells was discovered and proved in the study. A 15L photobioreactor with internal airlift loop, equipped with internal and external illumination was designed and established. The main properties and parameters of the photobioreactor was studied and measured. Experimental results showed that the photobioreactor was suitable for the high cell density cultivation of microalgae. Two different genera of cyanobacteria with different morphology, Anabaena sp. PCC7120 and Synechococcus sp. PCC7002 were selected and the autophototrophic cultivation of the cells were carried out successfully in the bioreactor. The effects of environmental and operated factors on cell growth were investigated during the cultivation. The preliminary basic laws of microalgae culture and optimal culture conditions were determined and finally reached a higher cell density was obtained. This work could establish the cornerstone in the culture of transgenic microalgae in the future. |
| 语种 | 中文 |
| 公开日期 | 2013-09-27 |
| 页码 | 78 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/1967]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | 康瑞娟. 蓝藻在光生物反应器中的光自养培养[D]. 中国科学院研究生院. 1999. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。