青霉素萃取体系溶剂流失机理研究
文献类型：学位论文
| 作者 | 张颖 |
| 学位类别 | 硕士 |
| 答辩日期 | 1999-07-20 |
| 授予单位 | 中国科学院研究生院 |
| 导师 | 刘会洲 |
| 关键词 | 表面活性剂 增溶作用 乙酸丁酯流失 青霉素 |
| 其他题名 | Study of Mechanism of Solvent Losses in the Extraction System of Penicillin |
| 中文摘要 | 本文用离子型表面活性剂和青霉素G的水溶液作水相，用乙酸丁酯作有机溶剂模拟了青霉素萃取体系，研究了不同条件下乙酸丁酯在水相中的滞留行为，发现有机溶剂在水相中的流失与表面活性剂在水中形成聚集体有关。在阴离子表面活性剂十二烷基硫酸钠（SDS）体系中，乙酸丁酯在水相中的浓度主要决定于SDS的浓度。当SDS超过其自身的临界胶束浓度时，SDS分子在水相形成聚集体，乙酸丁酯被增溶在这些聚集体中而大量进入水相。水相中青霉素C的存在、离子强度以及pH等因素都对该增溶作用有影响。在阳离子表面活性剂十六烷基三甲基溴化铵（CTAB）体系中，乙酸丁酯的流失同样由表面活性剂的聚集和增溶行为引起。CTAB的浓度、青霉素G的存在、离子强度和pH等因素都有影响，但是相互关系更为复杂。萃前水相中青霉素G的存在使溶剂的流失量急剧增加，有时也会引起萃取体系中具有双连续结构的第三相的生成。动态激光光散射结果证明了上述两种体系中聚集体的存在。红外光谱和拉曼光谱研究了以上表面活性剂增溶乙酸丁酯的体系，发现该体系中存在“表面”乙酸丁酯和“体相”乙酸丁酯两种形式的有机相分子。而且，乙酸丁酯增溶在胶团表面，并随着增溶量的增加而逐渐转移到胶团内核。 |
| 英文摘要 | The experimental modeling of solvent extraction of penicillin G from fermentation broth was carried out, with solutions of ionic surfactants as aqueous phase and butyl acetate as the organic solvent. The properties of butyl acetate remaining in the aqueous phases under different conditions were studied. It was found that three exists certain relationship between the aggregation of amphiphilic molecules and solvent losses. The concentrations of butyl acetate in the aqueous phases were determined with the addition of sodium dodecyl sulfate (SDS) in the anionic surfactant systems. When the concentrations of SDS used exceed its critical micelle concentration, the aggregation from of SDS are present in the aqueous phase with butyl acetate solubilized in aggregates, resulting to a large amount of solvent present in the aqueous phase. The presence of penicillin G, concentrations of inorganic salts and pH of the aqueous phase have a strong effect on the solubilization of butyl acetate in the aqueous phase. The loss of butyl acetate in the aqueous phase was found in the aggregates with addition of cationic surfactant such as cetyltrimethyl ammonium bromide (CTAB). The effects of inorganic salt, penicillin G and pH on solubilization of butyl acetate still can be found though more complicated. The solvent losses can be further increased with the formation of the third phase when the penicillin G is present in the system. Data obtained with dynamic laser scattering confirm that the surfactant aggregates are present in the two systems studied as described above. The solubilization system were also studied by FT-IR and Raman spectroscopy. Results show that there are tow forms of butyl acetate coexisting in the system: the "interface" butyl acetate and the "bulk" butyl acetate. Moreover, the molecules of butyl acetate are solubilized on the surface of micelles, and move gradually toward the cores of micelles with additional solubilization of butyl acetate. |
| 语种 | 中文 |
| 公开日期 | 2013-09-27 |
| 页码 | 78 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/1976]  |
| 专题 | 过程工程研究所_研究所（批量导入） |
| 推荐引用方式 GB/T 7714 | 张颖. 青霉素萃取体系溶剂流失机理研究[D]. 中国科学院研究生院. 1999. |
入库方式： OAI收割
来源：过程工程研究所
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