CRISPR/Cas12a-Derived electrochemical aptasensor for ultrasensitive detection of COVID-19 nucleocapsid protein
文献类型：期刊论文
| 作者 | Han, Cong3; Li, Wenjin3; Li, Qian2; Xing, Wenping2; Luo, Hang3; Ji, Haishuo2; Fang, Xiaona1; Luo, Zhaofeng1; Zhang, Liyun3 |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2022-03-15 |
| 卷号 | 200 |
| ISSN号 | 0956-5663 |
| 关键词 | COVID-19 Nucleocapsid protein CRISPR/Cas12a system Electrochemical aptasensor Aptamer |
| DOI | 10.1016/j.bios.2021.113922 |
| 通讯作者 | Zhang, Liyun(liyunzhang@nankai.edu.cn) |
| 英文摘要 | Fast, affordable, portable, and sensitive technology to detect COVID-19 is critical to address the current outbreak. Here, we present a CRISPR/Cas12a-derived electrochemical aptasensor for cost-effective, fast, and ultrasensitive COVID-19 nucleocapsid protein (Np) detection. First, an electrochemical sensing interface was fabricated by immobilizing methylene blue labeled poly adenines DNA sequence (polyA-MB electrochemical reporter) on a gold electrode surface. Second, an arched probe was prepared via hybridization of Np aptamer and an activator strand. In the presence of COVID-19 Np, the activator strand could be released from the arched probe due to the specific interaction between the target and the aptamer, which then activated the trans-cleavage activity of the CRISPR/Cas12a system. Subsequently, the polyA-MB reporters were cleaved from the electrode surface, decreasing the current of differential pulse voltammetry (DPV) at a potential of -0.27 V(vs. Ag/AgCl). The CRISPR/Cas12a-derived electrochemical aptasensor shows a highly efficient performance for COVID-19 Np detection in 50 pg mL(-1) to 100 ng mL(-1) with a limit of detection (LOD) low to 16.5 pg mL(-1). Notably, the whole process of one test can be completed within 30 min. Simultaneously, the aptasensor displays a high selectivity to other proteins. The further measurements demonstrate that the aptasensor is robust in a natural system for point-of-care testing, such as in tap water, milk, or serum. The aptasensor is universal and expandable and holds great potential in the COVID-19 early diagnosis, environmental surveillance, food security, and other aspects. |
| WOS关键词 | DNA ; CRISPR-CAS12A |
| 资助项目 | National Natural Science Foundation of China[22077069] ; Natural Science Foundation of Tianjin[19JCZDJC33400] ; Fundamental Research Funds for the Central Universities, Nankai University[63201111] ; Nankai University |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| WOS记录号 | WOS:000772286100008 |
| 资助机构 | National Natural Science Foundation of China ; Natural Science Foundation of Tianjin ; Fundamental Research Funds for the Central Universities, Nankai University ; Nankai University |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/128271]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Zhang, Liyun |
| 作者单位 | 1.Univ Chinese Acad Sci, Chinese Acad Sci, Aptamer Select Ctr, Inst Basic Med & Canc IBMC,Canc Hosp,Key Lab Zhej, Hangzhou 310022, Zhejiang, Peoples R China 2.Nankai Univ, Coll Pharm, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 3.Nankai Univ, Coll Life Sci, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Cong,Li, Wenjin,Li, Qian,et al. CRISPR/Cas12a-Derived electrochemical aptasensor for ultrasensitive detection of COVID-19 nucleocapsid protein[J]. BIOSENSORS & BIOELECTRONICS,2022,200. |
| APA | Han, Cong.,Li, Wenjin.,Li, Qian.,Xing, Wenping.,Luo, Hang.,...&Zhang, Liyun.(2022).CRISPR/Cas12a-Derived electrochemical aptasensor for ultrasensitive detection of COVID-19 nucleocapsid protein.BIOSENSORS & BIOELECTRONICS,200. |
| MLA | Han, Cong,et al."CRISPR/Cas12a-Derived electrochemical aptasensor for ultrasensitive detection of COVID-19 nucleocapsid protein".BIOSENSORS & BIOELECTRONICS 200(2022). |
入库方式： OAI收割
来源：合肥物质科学研究院
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