Scatter-hoarding rodents are important seed predators and dispersers in various forest ecosystems and significantly influence the seed fate and seedling regeneration of many tree species. Canopy openness is believed to have an important influence on the foraging behavior of scatter-hoarding rodents, mainly because of the variation in predation risk between open and canopy microsites. Most of the current studies have mainly focused on the spatial variation in canopy openness within a forest or on comparisons among forests; however, the question of how variation in canopy openness at the individual tree scale affects seed-rodent interaction has received little attention, although tree-to-tree variation in canopy openness is ubiquitous. In this study, we measured the individual canopy openness of 45 trees belonging to three species in a subtropical forest, and compared seed dispersal and predation by rodents under the selected trees by labeling and tracking 4,500 seeds. Our results showed that canopy openness differed among individual trees both within and across species. More importantly, our results proved that individual tree variation in canopy openness significantly affected seed dispersal and predation by rodents, although not all species followed a consistent pattern. Seeds under trees with larger canopy openness were more likely to be removed rather than eaten in situ and dispersed farther. Our study highlights the pattern that individual tree characteristics (e.g., canopy openness) have profound effects on rodent-mediated seed dispersal services, which may further lead to tree-to-tree variation in seed fate and seedling regeneration. Our results also provide important implications for forest conservation and management: crown pruning can promote seed dispersal by rodents and natural seedling regeneration.