Heat Shock 70 kDa Protein Cognate 3 of Brown Planthopper Is Required for Survival and Suppresses Immune Response in Plants
文献类型：期刊论文
| 作者 | Yang, Houhong1; Zhang, Xiaoya5; Li, Hanjing5; Ye, Yuxuan5; Li, Zhipeng1; Han, Xiao4; Hu, Yanru4 ; Zhang, Chuanxi3,5; Jiang, Yanjuan4
|
| 刊名 | INSECTS
 |
| 出版日期 | 2022 |
| 卷号 | 13期号:3页码:- |
| 关键词 | brown planthoppers NlHSC70-3 salivary protein immune response rice |
| ISSN号 | 2075-4450 |
| DOI | 10.3390/insects13030299 |
| 英文摘要 | Simple Summary Heat shock 70 kDa protein cognate 3 (NlHSC70-3) of brown planthopper (BPH) is important to the growth and development of BPH. However, if it also regulates plant physiological processes remain unclear. In this study, NlHSC70-3 is identified from a compared protein profile of Nipponbare tissues after BPH infestation. The predicted signal peptide and high levels of transcription in salivary glands and ovaries supported that it is a secreted protein. Function analysis demonstrates that NlHSC70-3 is required for the survival of BPH on rice. Plant immune responses, including flg22-induced ROS bursts and the expression of defense-related genes, are suppressed by NlHSC70-3 in Nicotiana benthamiana. Thus, NlHSC70-3 is secreted into rice by BPH and may interfere with plant defense responses to facilitate its survival. The brown planthopper (Nilaparvata lugens) is a monophagous pest of rice (Oryza sativa), which threatens food security around the world. Insect Heat shock proteins 70 kDa (Hsp70s) play a key role in insect growth and development, however, if they also modulate the plant physiological processes is still unclear. In this study, we identified the Heat shock 70 kDa protein cognate 3 (NlHSC70-3) of BPH from compared protein profiles of Nipponbare tissues after BPH infestation via LC/MS. NlHSC70-3 has a predicted signal peptide and displays high transcription levels in the salivary glands, which further supported that it is secreted into plants by BPH during the feeding process. Using RNA interference (RNAi), we showed that NlHSC70-3 is indispensable for the survival of BPH on rice. Most importantly, NlHSC70-3 mediates the plant immune responses including cell death, flg22-induced ROS burst and defense-related gene expression in N. benthamiana. These results demonstrate that NlHSC70-3 may function as an effector manipulating plant physiological processes to facilitate pest survival on rice, which provides a new potential target for future pest control. |
| 学科主题 | Entomology |
| 语种 | 英语 |
| WOS记录号 | WOS:000775218000001 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/12977]  |
| 专题 | 西双版纳热带植物园_2012年后新成立研究组 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Trop Plant Resources & Sustainable Use, Xishuangbanna Trop Bot Garden, Kunming 650223, Yunnan, Peoples R China 2.Ningbo Univ, Inst Plant Virol, Ningbo 315000, Peoples R China 3.Chinese Acad Sci, Ctr Econ Bot, Core Bot Gardens, Mengla 666303, Peoples R China 4.Zhejiang Univ, Inst Insect Sci, Hangzhou 310058, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Houhong,Zhang, Xiaoya,Li, Hanjing,et al. Heat Shock 70 kDa Protein Cognate 3 of Brown Planthopper Is Required for Survival and Suppresses Immune Response in Plants[J]. INSECTS,2022,13(3):-. |
| APA | Yang, Houhong.,Zhang, Xiaoya.,Li, Hanjing.,Ye, Yuxuan.,Li, Zhipeng.,...&Jiang, Yanjuan.(2022).Heat Shock 70 kDa Protein Cognate 3 of Brown Planthopper Is Required for Survival and Suppresses Immune Response in Plants.INSECTS,13(3),-. |
| MLA | Yang, Houhong,et al."Heat Shock 70 kDa Protein Cognate 3 of Brown Planthopper Is Required for Survival and Suppresses Immune Response in Plants".INSECTS 13.3(2022):-. |
入库方式： OAI收割
来源：西双版纳热带植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。