Genome-wide high-throughput signal peptide screening via plasmid pUC256E improves protease secretion in Lactiplantibacillus plantarum and Pediococcus acidilactici
文献类型：期刊论文
| 作者 | Chen, Binbin; Loo, Bryan Zong Lin; Cheng, Ying Ying; Song, Peng2; Fan, Huan3 ; Latypov, Oleg; Kittelmann, Sandra
|
| 刊名 | BMC GENOMICS
 |
| 出版日期 | 2022 |
| 卷号 | 23期号:_页码:- |
| ISSN号 | 1471-2164 |
| 关键词 | Signal peptide Protease secretion High-throughput screening Genetic engineering Lactiplantibacillus plantarum Pediococcus acidilactici |
| DOI | 10.1186/s12864-022-08292-3 |
| 英文摘要 | Background Proteases catalyze the hydrolysis of peptide bonds of proteins, thereby improving dietary protein digestibility, nutrient availability, as well as flavor and texture of fermented food and feed products. The lactobacilli Lactiplantibacillus plantarum (formerly Lactobacillus plantarum) and Pediococcus acidilactici are widely used in food and feed fermentations due to their broad metabolic capabilities and safe use. However, extracellular protease activity in these two species is low. Here, we optimized protease expression and secretion in L. plantarum and P. acidilactici via a genetic engineering strategy. Results To this end, we first developed a versatile and stable plasmid, pUC256E, which can propagate in both L. plantarum and P. acidilactici. We then confirmed expression and secretion of protease PepG1 as a functional enzyme in both strains with the aid of the previously described L. plantarum-derived signal peptide LP_0373. To further increase secretion of PepG1, we carried out a genome-wide experimental screening of signal peptide functionality. A total of 155 predicted signal peptides originating from L. plantarum and 110 predicted signal peptides from P. acidilactici were expressed and screened for extracellular proteolytic activity in the two different strains, respectively. We identified 12 L. plantarum signal peptides and eight P. acidilactici signal peptides that resulted in improved yield of secreted PepG1. No significant correlation was found between signal peptide sequence properties and its performance with PepG1. Conclusion The vector developed here provides a powerful tool for rapid experimental screening of signal peptides in both L. plantarum and P. acidilactici. Moreover, the set of novel signal peptides identified was widely distributed across strains of the same species and even across some closely related species. This indicates their potential applicability also for the secretion of other proteins of interest in other L. plantarum or P. acidilactici host strains. Our findings demonstrate that screening a library of homologous signal peptides is an attractive strategy to identify the optimal signal peptide for the target protein, resulting in improved protein export. |
| 学科主题 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000742000700001 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/13020]  |
| 专题 | 西双版纳热带植物园_2012年后新成立研究组 |
| 作者单位 | 1.Chinese Acad Sci, Ctr Integrat Conservat, Xishuangbanna Trop Bot Garden, Menglun, Yunnan, Peoples R China 2.Natl Univ Singapore, Ctr Translat Med, WIL NUS Corp Lab, Wilmar Int Ltd, Singapore, Singapore 3.Wilmar Shanghai Biotechnol Res & Dev Ctr Co Ltd, Wilmar Int Ltd, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Binbin,Loo, Bryan Zong Lin,Cheng, Ying Ying,et al. Genome-wide high-throughput signal peptide screening via plasmid pUC256E improves protease secretion in Lactiplantibacillus plantarum and Pediococcus acidilactici[J]. BMC GENOMICS,2022,23(_):-. |
| APA | Chen, Binbin.,Loo, Bryan Zong Lin.,Cheng, Ying Ying.,Song, Peng.,Fan, Huan.,...&Kittelmann, Sandra.(2022).Genome-wide high-throughput signal peptide screening via plasmid pUC256E improves protease secretion in Lactiplantibacillus plantarum and Pediococcus acidilactici.BMC GENOMICS,23(_),-. |
| MLA | Chen, Binbin,et al."Genome-wide high-throughput signal peptide screening via plasmid pUC256E improves protease secretion in Lactiplantibacillus plantarum and Pediococcus acidilactici".BMC GENOMICS 23._(2022):-. |
入库方式： OAI收割
来源：西双版纳热带植物园
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