Cold Response Transcriptome Analysis of the Alternative Splicing Events Induced by the Cold Stress in D. catenatum
文献类型：期刊论文
| 作者 | Zheng, Yan; Luo, Landi1; Chen, Qian; Yang, Danni4; Gong, Yuqiang3; Yang, Ya; Qin, Xiangshi; Wang, Yuhua; Kong, Xiangxiang; Yang, Yongping1
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| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| 出版日期 | 2022 |
| 卷号 | 23期号:1页码:- |
| ISSN号 | 1422-0067 |
| 关键词 | Dendrobium catenatum Lindl alternative splicing cold stress DcCBP20 |
| DOI | 10.3390/ijms23020981 |
| 英文摘要 | Dendrobium catenatum Lindl is a valuable medicinal herb and gardening plant due to its ornamental value and special medical value. Low temperature is a major bottleneck restricting D. catenatum expansion towards the north, which influences the quality and yield of D. catenatum. In this study, we analysed the cold response of D. catenatum by RNA-Seq. A total of 4302 differentially expressed genes were detected under cold stress, which were mainly linked to protein kinase activity, membrane transport and the glycan biosynthesis and metabolism pathway. We also identified 4005 differential alternative events in 2319 genes significantly regulated by cold stress. Exon skipping and intron retention were the most common alternative splicing isoforms. Numerous genes were identified that differentially modulated under cold stress, including cold-induced transcription factors and splicing factors mediated by AS (alternative splicing). GO enrichment analysis found that differentially alternatively spliced genes without differential expression levels were related to RNA/mRNA processing and spliceosomes. DAS (differentially alternative splicing) genes with different expression levels were mainly enriched in protein kinase activity, plasma membrane and cellular response to stimulus. We further identified and cloned DcCBP20 in D. catenatum; we found that DcCBP20 promotes the generation of alternative splicing variants in cold-induced genes under cold stress via genetic experiments and RT-PCR. Taken together, our results identify the main cold-response pathways and alternative splicing events in D. catenatum responding to cold treatment and that DcCBP20 of D. catenatum get involved in regulating the AS and gene expression of cold-induced genes during this process. Our study will contribute to understanding the role of AS genes in regulating the cold stress response in D. catenatum. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000749793200001 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/13034]  |
| 专题 | 西双版纳热带植物园_2012年后新成立研究组 |
| 作者单位 | 1.Chinese Acad Sci, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 2.Yunnan Agr Univ, Coll Hort & Landscape, Kunming 650500, Yunnan, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Xishuangbanna Trop Bot Garden, CAS Lab Trop Plant Resources & Sustainable Use, Menglun 666303, Mengla, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Yan,Luo, Landi,Chen, Qian,et al. Cold Response Transcriptome Analysis of the Alternative Splicing Events Induced by the Cold Stress in D. catenatum[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(1):-. |
| APA | Zheng, Yan.,Luo, Landi.,Chen, Qian.,Yang, Danni.,Gong, Yuqiang.,...&Yang, Yongping.(2022).Cold Response Transcriptome Analysis of the Alternative Splicing Events Induced by the Cold Stress in D. catenatum.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(1),-. |
| MLA | Zheng, Yan,et al."Cold Response Transcriptome Analysis of the Alternative Splicing Events Induced by the Cold Stress in D. catenatum".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.1(2022):-. |
入库方式： OAI收割
来源：西双版纳热带植物园
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