Differential regulation of stiffness, topography, and dimension of substrates in rat mesenchymal stem cells
文献类型：期刊论文
| 作者 | Li Z(李展) ; Gong YW(宫元卫); Sun SJ(孙树津); Du Y(杜宇); Lv DY(吕东媛); Liu XF(刘晓锋); Long M(龙勉)
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| 刊名 | BIOMATERIALS
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| 出版日期 | 2013-10 |
| 卷号 | 34期号:31页码:7616-7625 |
| 通讯作者邮箱 | sunshj@imech.ac.cn; mlong@imech.ac.cn |
| 关键词 | Stiffness Topography Dimension Stem cell Proliferation Differentiation |
| ISSN号 | 0142-9612 |
| 产权排序 | [Li, Zhan;Gong, Yuanwei;Sun, Shujin;Du, Yu;Lu, Dongyuan;Liu, Xiaofeng;Long, Mian] Chinese Acad Sci, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China; Chinese Acad Sci, Key Lab Micrograv, Nat Micrograv Lab, Inst Mech, Beijing 100190, Peoples R China |
| 通讯作者 | Sun, SJ (reprint author), Chinese Acad Sci, Ctr Biomech & Bioengn, Beijing 100190, Peoples R China. |
| 中文摘要 | The physiological microenvironment of the stem cell niche, including the three factors of stiffness, topography, and dimension, is crucial to stem cell proliferation and differentiation. Although a growing body of evidence is present to elucidate the importance of these factors individually, the interaction of the biophysical parameters of the factors remains insufficiently characterized, particularly for stem cells. To address this issue fully, we applied a micro-fabricated polyacrylamide hydrogel substrate with two elasticities, two topographies, and three dimensions to systematically test proliferation, morphology and spreading, differentiation, and cytoskeletal re-organization of rat bone marrow mesenchymal stem cells (rBMSCs) on twelve cases. An isolated but not combinatory impact of the factors was found regarding the specific functions. Substrate stiffness or dimension is predominant in regulating cell proliferation by fostering cell growth on stiff, unevenly dimensioned substrate. Topography is a key factor for manipulating cell morphology and spreading via the formation of a large spherical shape in a pillar substrate but not in a grooved substrate. Although stiffness leads to osteogenic or neuronal differentiation of rBMSCs on a stiff or soft substrate, respectively, topography or dimension also plays a lesser role in directing cell differentiation. Neither an isolated effect nor a combinatory effect was found for actin or tubulin expression, whereas a seemingly combinatory effect of topography and dimension was found in manipulating vimentin expression. These results further the understandings of stem cell proliferation, morphology, and differentiation in a physiologically mimicking microenvironment. |
| 学科主题 | 生物力学 |
| 分类号 | 一类 |
| 收录类别 | SCI ; EI |
| 资助信息 | National Natural Science Foundation of China [31110103918]; National Key Basic Research Foundation of China [2011CB710904]; Strategic Priority Research Program [XDA01030102]; National High Technology Research and Development Program of China [2011AA020109] |
| 原文出处 | http://dx.doi.org/10.1016/j.biomaterials.2013.06.059 |
| 语种 | 英语 |
| WOS记录号 | WOS:000323459100006 |
| 公开日期 | 2013-09-27 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/47445]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Li Z,Gong YW,Sun SJ,et al. Differential regulation of stiffness, topography, and dimension of substrates in rat mesenchymal stem cells[J]. BIOMATERIALS,2013,34(31):7616-7625. |
| APA | Li Z.,Gong YW.,Sun SJ.,Du Y.,Lv DY.,...&Long M.(2013).Differential regulation of stiffness, topography, and dimension of substrates in rat mesenchymal stem cells.BIOMATERIALS,34(31),7616-7625. |
| MLA | Li Z,et al."Differential regulation of stiffness, topography, and dimension of substrates in rat mesenchymal stem cells".BIOMATERIALS 34.31(2013):7616-7625. |
入库方式： OAI收割
来源：力学研究所
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