Structural Characterization and Heparanase Inhibitory Activity of Fucosylated Glycosaminoglycan from Holothuria floridana
文献类型：期刊论文
| 作者 | Shi,Xiang; Guan,Ruowei; Zhou,Lutan; Zuo,Zhichuang; Tao,Xuelin; Wang,Pin; Zhou,Yanrong; Yin,Ronghua; Zhao,Longyan ; Gao,Na
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| 刊名 | MARINE DRUGS
 |
| 出版日期 | 2021 |
| 卷号 | 19期号:3页码:162 |
| ISSN号 | 1660-3397 |
| 关键词 | Holothuria floridana fucosylated glycosaminoglycans oligosaccharide chemical structure heparanase |
| DOI | 10.3390/md19030162 |
| 英文摘要 | Unique fucosylated glycosaminoglycans (FG) have attracted increasing attention for various bioactivities. However, the precise structures of FGs usually vary in a species-specific manner. In this study, HfFG was isolated from Holothuria floridana and purified by anion exchange chromatography with the yield of similar to 0.9%. HfFG was composed of GlcA, GalNAc and Fuc, its molecular weight was 47.3 kDa, and the -OSO3-/-COO- molar ratio was 3.756. HfFG was depolymerized by a partial deacetylation-deaminative cleavage method to obtain the low-molecular-weight HfFG (dHfFG). Three oligosaccharide fragments (Fr-1, Fr-2, Fr-3) with different molecular weights were isolated from the dHfFG, and their structures were revealed by 1D and 2D NMR spectroscopy. HfFG should be composed of repeating trisaccharide units -{(L-FucS-alpha 1,3-)D-GlcA-beta 1,3-D-GalNAc(4S6S)-beta 1,4-}-, in which sulfated fucose (FucS) includes Fuc(2S4S), Fuc(3S4S) and Fuc(4S) residues linked to O-3 of GlcA in a ratio of 45:35:20. Furthermore, the heparanase inhibitory activities of native HfFG and oligosaccharide fragments (Fr-1, Fr-2, Fr-3) were evaluated. The native HfFG and its oligosaccharides exhibited heparanase inhibitory activities, and the activities increased with the increase of molecular weight. Additionally, structural characteristics such as sulfation patterns, the terminal structure of oligosaccharides and the presence of fucosyl branches may be important factors affecting heparanase inhibiting activity. |
| WOS记录号 | WOS:000633845000001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/73024]  |
| 专题 | 中国科学院昆明植物研究所 |
| 作者单位 | 1.Guangxi Univ Chinese Med, Inst Marine Drugs, Nanning 530200, Peoples R China 2.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan 430074, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 4.South Cent Univ Nationalities, Natl Demonstrat Ctr Expt Ethnopharmacol Educ, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi,Xiang,Guan,Ruowei,Zhou,Lutan,et al. Structural Characterization and Heparanase Inhibitory Activity of Fucosylated Glycosaminoglycan from Holothuria floridana[J]. MARINE DRUGS,2021,19(3):162. |
| APA | Shi,Xiang.,Guan,Ruowei.,Zhou,Lutan.,Zuo,Zhichuang.,Tao,Xuelin.,...&Zhao,Jinhua.(2021).Structural Characterization and Heparanase Inhibitory Activity of Fucosylated Glycosaminoglycan from Holothuria floridana.MARINE DRUGS,19(3),162. |
| MLA | Shi,Xiang,et al."Structural Characterization and Heparanase Inhibitory Activity of Fucosylated Glycosaminoglycan from Holothuria floridana".MARINE DRUGS 19.3(2021):162. |
入库方式： OAI收割
来源：昆明植物研究所
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