High-molecular-weight fucosylated glycosaminoglycan induces human platelet aggregation depending on alpha(IIb)beta(3) and platelet secretion
文献类型：期刊论文
| 作者 | Lin,Lisha; Yang,Lian; Chen,Jun; Zhou,Lutan; Li,Sujuan; Gao,Na; Zhao,Jinhua
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| 刊名 | PLATELETS
 |
| 出版日期 | 2021 |
| 卷号 | 32期号:7页码:975-983 |
| ISSN号 | 0953-7104 |
| 关键词 | Aggregation fucosylated glycosaminoglycan human platelets secretion alpha(IIb beta 3) DEPOLYMERIZED HOLOTHURIAN GLYCOSAMINOGLYCAN INTRINSIC TENASE COMPLEX INTEGRIN ALPHA(IIB)BETA(3) ACTIVATION AGGLUTINATION RECEPTOR ADP INHIBITION GENERATION MECHANISM |
| DOI | 10.1080/09537104.2020.1820976 |
| 英文摘要 | Fucosylated glycosaminoglycan (FG) from sea cucumbers has been reported to have anticoagulant effects via targeting intrinsic tenase. However, FG from natural source also potentially poses risks due to its FXIIa activation and platelet aggregating effects. Here, we found that the effect of FG on human platelet aggregation depended on both the sulfation pattern and chain length. FGs with higher content of Fuc(2S4S) and larger molecular weight (>= 14 kD) had stronger activity. Both platelet aggregation and P-selectin expression induced by TaFG (an FG fromThelenota ananas) were decreased as the molecular weight reduced. Ticagrelor, aspirin and wortmannin completely blocked the secretion (ADP) but only partially blocked the aggregation induced by TaFG. Tirofiban an alpha(IIb)beta(3) antagonist however potently inhibited both the secretion and aggregation, with IC50 of 6.01 +/- 1.1.97 nM. Furthermore, TaFG could bind to human alpha(IIb)beta(3) with high affinity, and the affinities of two FGs were paralleled with their activity in platelet aggregation or activation. These results indicated that alpha(IIb)beta(3) played an important role in TaFG-induced platelet aggregation which was mediated by PI3K, and that platelet secretion was required for the amplification of aggregation. |
| WOS记录号 | WOS:000572529300001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/73028]  |
| 专题 | 中国科学院昆明植物研究所 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 3.Yunnan Univ, Sch Chem Sci & Technol, Kunming 650091, Yunnan, Peoples R China 4.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin,Lisha,Yang,Lian,Chen,Jun,et al. High-molecular-weight fucosylated glycosaminoglycan induces human platelet aggregation depending on alpha(IIb)beta(3) and platelet secretion[J]. PLATELETS,2021,32(7):975-983. |
| APA | Lin,Lisha.,Yang,Lian.,Chen,Jun.,Zhou,Lutan.,Li,Sujuan.,...&Zhao,Jinhua.(2021).High-molecular-weight fucosylated glycosaminoglycan induces human platelet aggregation depending on alpha(IIb)beta(3) and platelet secretion.PLATELETS,32(7),975-983. |
| MLA | Lin,Lisha,et al."High-molecular-weight fucosylated glycosaminoglycan induces human platelet aggregation depending on alpha(IIb)beta(3) and platelet secretion".PLATELETS 32.7(2021):975-983. |
入库方式： OAI收割
来源：昆明植物研究所
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