An Improved Protocol for Asymbiotic Seed Germination and Seedling Development of Paphiopedilum tigrinum
文献类型：期刊论文
| 作者 | Yao,Linling; Huang,Jialin ; Zhang,Shibao
|
| 刊名 | HORTICULTURAE
 |
| 出版日期 | 2021 |
| 卷号 | 7期号:9页码:298 |
| 关键词 | basal media endangered orchid germplasm conservation and utilization in vitro propagation IN-VITRO PROPAGATION LADYS SLIPPER ORCHID ACTIVATED-CHARCOAL PLANT-REGENERATION CULTURE MULTIPLICATION ESTABLISHMENT DELENATII MEDIA |
| ISSN号 | 2311-7524 |
| DOI | 10.3390/horticulturae7090298 |
| 英文摘要 | Paphiopedilum tigrinum is an endangered orchid with high ornamental value. However, seed germination and seedling regeneration in P. tigrinum is very difficult in vitro. Little is known about why P. tigrinum seedlings are difficult to propagate or how to improve the seed germination and seedling rates of this species. In this study, we investigated the developmental process of P. tigrinum from asymbiotic seed germination to seedling rooting by comparing it with P. appletoniantum, a much easier species for germination and seedling formation. We found that asymbiotic seed germination in P. tigrinum is limited by severe browning of the protocorm at the seed germination stage, and protocorm rooting at the differentiation stage was also proved to be difficult. The optimal medium for seed germination of P. tigrinum was a modified Harvais (mHa) medium supplemented with 0.5 mg center dot L-1 kinetin (Kin), 0.1 g center dot L-1 activated charcoal (AC) and 100 mL center dot L-1 coconut water (CW). At the protocorm differentiation stage, seedlings with 1-2 leaves were obtained on a 1/4 MS medium supplemented with 1.0 mg center dot L-1 6-benzylaminopurin (BA), 0.3 g center dot L-1 AC and 50-100 mL center dot L-1 CW after culturing for 120 day. At the seedling subculture stage, a 1/2 MS medium supplemented with 0.5-1.5 g center dot L-1 AC and 100 mL center dot L-1 CW was better for leaf and root growth of P. tigrinum. At the rooting stage, a 1/2 MS medium supplemented with 1.0 g center dot L-1 AC, 0.5 g center dot L-1 dolomite flour, 15 g center dot L-1 potato homogenate and 30 g center dot L-1 banana homogenate was most suitable for the growth and rooting of seedlings. This study has established an effective protocol for seed germination and seedling regeneration of P. tigrinum. |
| WOS记录号 | WOS:000699605400001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/73067]  |
| 专题 | 中国科学院昆明植物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China 2.Yuxi Normal Univ, Sch Chem Biol & Environm, Yuxi 653100, Peoples R China 3.Univ Chinese Acad Sci, Acad Life Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao,Linling,Huang,Jialin,Zhang,Shibao. An Improved Protocol for Asymbiotic Seed Germination and Seedling Development of Paphiopedilum tigrinum[J]. HORTICULTURAE,2021,7(9):298. |
| APA | Yao,Linling,Huang,Jialin,&Zhang,Shibao.(2021).An Improved Protocol for Asymbiotic Seed Germination and Seedling Development of Paphiopedilum tigrinum.HORTICULTURAE,7(9),298. |
| MLA | Yao,Linling,et al."An Improved Protocol for Asymbiotic Seed Germination and Seedling Development of Paphiopedilum tigrinum".HORTICULTURAE 7.9(2021):298. |
入库方式： OAI收割
来源：昆明植物研究所
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