NaKTI2, a Kunitz trypsin inhibitor transcriptionally regulated by NaWRKY3 and NaWRKY6, is required for herbivore resistance inNicotiana attenuata
文献类型：期刊论文
| 作者 | Yin,Min; Song,Na; Chen,Suiyun; Wu,Jinsong |
| 刊名 | PLANT CELL REPORTS
 |
| 出版日期 | 2021 |
| 卷号 | 40期号:1页码:97-109 |
| 关键词 | Nicotiana attenuata Kunitz trypsin inhibitor Alternaria alternata Spodoptera litura WRKY NICOTIANA-ATTENUATA PROTEINASE-INHIBITOR PHYTOALEXIN SCOPOLETIN ALTERNARIA-ALTERNATA ANTIFUNGAL ACTIVITY DEFENSE RESPONSES PLANT-RESPONSES TOBACCO VIRUS GENE |
| ISSN号 | 0721-7714 |
| DOI | 10.1007/s00299-020-02616-x |
| 英文摘要 | Key message Here, we reported that a pathogen- and herbivore-induced Kunitz trypsin inhibitor gene, NaKTI2, is required for herbivore resistance, and transcriptionally regulated mainly by NaWRKY3 and NaWRKY6 but not Jasmonate signaling. Plant protease inhibitor (PI) occurs widely in plant species, and is considered as an important part of plant defense arsenal against herbivores. Transcriptome analysis ofNicotiana attenuataleaves revealed that a Kunitz trypsin inhibitor gene,NaKTI2, was highly elicited after inoculation ofAlternaria alternata(tobacco pathotype). However, the roles ofNaKTI2in pathogen- and herbivore resistance and its regulation were unclear. NaKTI2 had typical domains of Kunitz trypsin inhibitors and exhibited a high level of trypsin protease inhibitor activities when transiently over-expressed. The transcripts ofNaKTI2could be induced byA. alternataandSpodoptera lituraoral secretions (OS). SilencingNaKTI2via virus-induced gene silencing technique has no influence on lesion diameters developed onN. attenuataleaves afterA. alternatainoculation, butS. lituralarvae gained more mass and had higher survivorship onNaKTI2-silenced plants. Meanwhile, the expression ofNaPI, a PI gene essential for herbivore resistance previously identified inN. attenuata, was not affected inNaKTI2-silenced plants. UnlikeNaPI, which was predominantly regulated by jasmonate (JA) signaling, OS-elicitedNaKTI2transcripts were only slightly reduced in JA-deficient plants, but were dramatically decreased inNaWRKY3- andNaWRKY6- silenced plants, respectively. Further electromobility shift assays indicated that NaWRKY3 and NaWRKY6 could directly bind to the promoter regions ofNaKTI2in vitro. Taken together, our results demonstrate that in addition toNaPI,NaKTI2, a pathogen- and herbivore-induced Kunitz trypsin inhibitor gene, is also required for herbivore resistance, and mainly regulated by NaWRKY3 and NaWRKY6. |
| WOS记录号 | WOS:000578104000001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/73207]  |
| 专题 | 中国科学院昆明植物研究所 |
| 作者单位 | 1.Yunnan Univ, Biocontrol Engn Res Ctr Crop Dis & Pest, Sch Life Sci, Kunming 650091, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 10049, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Fungal Divers & Green Dev, Kunming 650201, Yunnan, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin,Min,Song,Na,Chen,Suiyun,et al. NaKTI2, a Kunitz trypsin inhibitor transcriptionally regulated by NaWRKY3 and NaWRKY6, is required for herbivore resistance inNicotiana attenuata[J]. PLANT CELL REPORTS,2021,40(1):97-109. |
| APA | Yin,Min,Song,Na,Chen,Suiyun,&Wu,Jinsong.(2021).NaKTI2, a Kunitz trypsin inhibitor transcriptionally regulated by NaWRKY3 and NaWRKY6, is required for herbivore resistance inNicotiana attenuata.PLANT CELL REPORTS,40(1),97-109. |
| MLA | Yin,Min,et al."NaKTI2, a Kunitz trypsin inhibitor transcriptionally regulated by NaWRKY3 and NaWRKY6, is required for herbivore resistance inNicotiana attenuata".PLANT CELL REPORTS 40.1(2021):97-109. |
入库方式： OAI收割
来源：昆明植物研究所
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