High-content screening of diterpenoids from Isodon species as autophagy modulators and the functional study of their antiviral activities
文献类型：期刊论文
| 作者 | Huang,Lihong; Fu,Qiang; Dai,Jia-Meng; Yan,Bing-Chao; Wang,Dawei; Puno,Pema-Tenzin; Yue,Jianbo |
| 刊名 | CELL BIOLOGY AND TOXICOLOGY
 |
| 出版日期 | 2021 |
| 卷号 | 37期号:5页码:695-713 |
| ISSN号 | 0742-2091 |
| 关键词 | Diterpenoids Autophagy Lysosome Autophagosome Enanderinanin J Dengue virus (DENV) Zika virus (ZIKV) Japanese encephalitis virus (JEV) Enterovirus-A71 (EV-A71) |
| DOI | 10.1007/s10565-021-09580-6 |
| 英文摘要 | Autophagy is a conserved lysosomal degradation process, and abnormal autophagy has been associated with various pathological processes, e.g., neurodegeneration, cancer, and pathogen infection. Small chemical modulators of autophagy show the potential to treat autophagy-associated diseases. Diterpenoids, nature products found in various plants, exhibit a wide range of bioactivity, and we have recently isolated and characterized over 150 diterpenoids from Isodon species distributed in China. Here, we applied a high-content fluorescence imaging-based assay to assess these diterpenoids' ability to affect autophagic flux in HeLa cells. We found that enanderinanin J, an ent-kauranoid dimer, is an autophagy inhibitor, manifested by its ability to increase lysosomal pH and inhibit the fusion between autophagosomes and lysosomes. Autophagy has been shown to be either positively or negatively involved in the life cycle of Zika virus (ZIKV), Japanese encephalitis virus (JEV), Dengue virus (DENV), and enterovirus-A71 (EV-A71). We found that enanderinanin J significantly inhibited the infection of ZIKV, DENV, JEV, or EV-A71. Interestingly, although ATG5 knockdown inhibited ZIKV or JEV infection, enanderinanin J further inhibited the infection of ZIKV or JEV in ATG5-knockdown cells. Taken together, our data indicate that enanderinanin J inhibits autophagosome-lysosome fusion and is a potential antiviral agent. |
| WOS记录号 | WOS:000610495300001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/73368]  |
| 专题 | 中国科学院昆明植物研究所 |
| 作者单位 | 1.City Univ Hong Kong, Chengdu Res Inst, Chengdu, Peoples R China 2.Yunnan Key Lab Nat Med Chem, Kunming 650201, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Peoples R China 4.Xinjiang Agr Univ, Coll Vet Med, Urumqi, Peoples R China 5.City Univ Hong Kong, Dept Biomed Sci, Hong Kong, Peoples R China 6.City Univ Hong Kong, Shenzhen Res Inst, Shenzhen, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang,Lihong,Fu,Qiang,Dai,Jia-Meng,et al. High-content screening of diterpenoids from Isodon species as autophagy modulators and the functional study of their antiviral activities[J]. CELL BIOLOGY AND TOXICOLOGY,2021,37(5):695-713. |
| APA | Huang,Lihong.,Fu,Qiang.,Dai,Jia-Meng.,Yan,Bing-Chao.,Wang,Dawei.,...&Yue,Jianbo.(2021).High-content screening of diterpenoids from Isodon species as autophagy modulators and the functional study of their antiviral activities.CELL BIOLOGY AND TOXICOLOGY,37(5),695-713. |
| MLA | Huang,Lihong,et al."High-content screening of diterpenoids from Isodon species as autophagy modulators and the functional study of their antiviral activities".CELL BIOLOGY AND TOXICOLOGY 37.5(2021):695-713. |
入库方式： OAI收割
来源：昆明植物研究所
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