Development of a LC-HRMS based approach to boost structural annotation of isomeric citrus flavanones
文献类型：期刊论文
| 作者 | He,Ying-Jie; Zhou,Ying; Qin,Yan; Zhou,Zhong-Shun; Zhu,Meng; Zhu,Yan-Yan; Wang,Zhao-Jie; Xie,Tian-Zhen; Zhao,Li-Xing; Luo,Xiao-Dong
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| 刊名 | PHYTOCHEMICAL ANALYSIS
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| 出版日期 | 2021 |
| 卷号 | 32期号:5页码:749-756 |
| 关键词 | citrus flavanones collision energy fragmentation pattern high‐ resolution mass spectrometry ion ratio RESOLUTION MASS-SPECTROMETRY HESPERETIN ENANTIOMERS QUANTIFICATION NARINGENIN |
| ISSN号 | 0958-0344 |
| DOI | 10.1002/pca.3020 |
| 英文摘要 | Introduction The structural annotation of target relies on high-resolution mass spectrometry (HRMS) information resulting in dubious identities in most cases. The accurate annotation of isomeric structures is still challenging to be confirmed with significant bottleneck.Objective This study focused on the improvement of structural annotation of candidate structures via four pairs of isomeric flavanone-7-O-diglucosides and their basic flavanone aglycones commonly detected in citrus products.Method An integrated liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS) approach merging retention time, accurate mass, tandem mass spectrometry (MS/MS) information (diagnostic ions), ion ratio at selected collision energy was established successfully.Results Feasibility of this approach was validated confidently in biological samples with relative standard deviation (RSD) of ion ratio range from 3.91 to 12.28%. Differences of fragmentation patterns of citrus flavanones were illustrated reasonably. MS/MS fragments of (S)-hesperetin and (S)-isosakuranetin were complicated and showed typical radical ion [(1,2)A - H](center dot-) (m/z 164) in negative ESI mode due to the methoxyl group on B-ring, which showed huge difference with (R)-hesperetin and (R)-isosakuranetin.Conclusion This study integrated multiple levels to boost the confidence of structural annotation relied on LC-HRMS, and provided important values in practice for precise identification of citrus flavanones in biological matrices. |
| WOS记录号 | WOS:000599150100001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/73423]  |
| 专题 | 中国科学院昆明植物研究所 |
| 作者单位 | 1.Zhou, Zhong-Shun 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Yunnan, Peoples R China 3.Yunnan Univ, Sch Chem Sci & Technol, Kunming 650091, Yunnan, Peoples R China 4.Yunnan Univ, Yunnan Prov Ctr Res & Dev Nat Prod, Kunming 650091, Yunnan, Peoples R China 5.Yunnan Univ, Minist Educ, Key Lab Med Chem Nat Resource, Kunming 650091, Yunnan, Peoples R China 6.Zhao, Li-Xing 7.Xie, Tian-Zhen 8.Wang, Zhao-Jie 9.Zhu, Yan-Yan 10.[He, Ying-Jie |
| 推荐引用方式 GB/T 7714 | He,Ying-Jie,Zhou,Ying,Qin,Yan,et al. Development of a LC-HRMS based approach to boost structural annotation of isomeric citrus flavanones[J]. PHYTOCHEMICAL ANALYSIS,2021,32(5):749-756. |
| APA | He,Ying-Jie.,Zhou,Ying.,Qin,Yan.,Zhou,Zhong-Shun.,Zhu,Meng.,...&Luo,Xiao-Dong.(2021).Development of a LC-HRMS based approach to boost structural annotation of isomeric citrus flavanones.PHYTOCHEMICAL ANALYSIS,32(5),749-756. |
| MLA | He,Ying-Jie,et al."Development of a LC-HRMS based approach to boost structural annotation of isomeric citrus flavanones".PHYTOCHEMICAL ANALYSIS 32.5(2021):749-756. |
入库方式： OAI收割
来源：昆明植物研究所
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