Novel Cyclic Peptides from Lethal Amanita Mushrooms through a Genome-Guided Approach
文献类型：期刊论文
| 作者 | Zhou,Shengwen; Li,Xincan; Luli,Yunjiao; Li,Xuan; Chen,Zuo H.; Yuan,Pengcheng; Yang,Zhu L.; Li,Guohong; Luo,Hong |
| 刊名 | JOURNAL OF FUNGI
 |
| 出版日期 | 2021 |
| 卷号 | 7期号:3页码:204 |
| 关键词 | poisonous mushroom genome MSDIN family Amanitin LC– HRMS |
| ISSN号 | 2309-608X |
| DOI | 10.3390/jof7030204 |
| 英文摘要 | Most species in the genus Amanita are ectomycorrhizal fungi comprising both edible and poisonous mushrooms. Some species produce potent cyclic peptide toxins, such as alpha-amanitin, which places them among the deadliest organisms known to mankind. These toxins and related cyclic peptides are encoded by genes of the MSDIN family (named after the first five amino acid residues of the precursor peptides), and it is largely unknown to what extent these genes are expressed in the basidiocarps. In the present study, Amanita rimosa and Amanita exitialis were sequenced through the PacBio and Illumina techniques. Together with our two previously sequenced genomes, Amanita subjunquillea and Amanita pallidorosea, in total, 46 previously unknown MSDIN genes were discovered. The expression of over 80% of the MSDIN genes was demonstrated in A. subjunquillea. Through a combination of genomics and mass spectrometry, 12 MSDIN genes were shown to produce novel cyclic peptides. To further confirm the results, three of the cyclic peptides were chemically synthesized. The tandem mass spectrometry (MS/MS) spectra of the natural and the synthetic peptides shared a majority of the fragment ions, demonstrating an identical structure between each peptide pair. Collectively, the results suggested that the genome-guided approach is reliable for identifying novel cyclic peptides in Amanita species and that there is a large peptide reservoir in these mushrooms. |
| WOS记录号 | WOS:000633768200001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/73433]  |
| 专题 | 中国科学院昆明植物研究所 |
| 作者单位 | 1.Yunnan Univ, Sch Life Sci, Kunming 650091, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, CAS Key Lab Plant Div & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Fungal Div & Green Dev, Kunming 650201, Yunnan, Peoples R China 4.Chen, Zuo H.] Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Kunming Univ Sci & Technol, Dept Environm Sci & Engn, Kunming 650091, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou,Shengwen,Li,Xincan,Luli,Yunjiao,et al. Novel Cyclic Peptides from Lethal Amanita Mushrooms through a Genome-Guided Approach[J]. JOURNAL OF FUNGI,2021,7(3):204. |
| APA | Zhou,Shengwen.,Li,Xincan.,Luli,Yunjiao.,Li,Xuan.,Chen,Zuo H..,...&Luo,Hong.(2021).Novel Cyclic Peptides from Lethal Amanita Mushrooms through a Genome-Guided Approach.JOURNAL OF FUNGI,7(3),204. |
| MLA | Zhou,Shengwen,et al."Novel Cyclic Peptides from Lethal Amanita Mushrooms through a Genome-Guided Approach".JOURNAL OF FUNGI 7.3(2021):204. |
入库方式： OAI收割
来源：昆明植物研究所
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