A Cryptic Plant Terpene Cyclase Producing Unconventional 18-and 14-Membered Macrocyclic C-25 and C-20 Terpenoids with Immunosuppressive Activity
文献类型：期刊论文
| 作者 | Chen,Yue-Gui; Li,De-Sen; Ling,Yi; Liu,Yan-Chun; Zuo,Zhi-Li; Gan,Li-She; Luo,Shi-Hong; Hua,Juan; Chen,Ding-Yuan; Xu,Fan |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2021 |
| 卷号 | 60期号:48页码:25468-25476 |
| 关键词 | enzyme engineering immunosuppressive activity Leucosceptrum canum macrocyclic terpenoids sesterterpene synthase GERANYLFARNESYL DIPHOSPHATE SYNTHASE GLANDULAR TRICHOMES LEUCOSCEPTRUM-CANUM SCALE INSECT SESTERTERPENOIDS CONVERSION EVOLUTION PRECURSOR HARBOR |
| ISSN号 | 1433-7851 |
| DOI | 10.1002/anie.202110842 |
| 英文摘要 | A versatile terpene synthase (LcTPS2) producing unconventional macrocyclic terpenoids was characterized from Leucosceptrum canum. Engineered Escherichia coli and Nicotiana benthamiana expressing LcTPS2 produced six 18-/14-membered sesterterpenoids including five new ones and two 14-membered diterpenoids. These products represent the first macrocyclic sesterterpenoids from plants and the largest sesterterpenoid ring system identified to date. Two variants F516A and F516G producing approximately 3.3- and 2.5-fold, respectively, more sesterterpenoids than the wild-type enzyme were engineered. Both 18- and 14-membered ring sesterterpenoids displayed significant inhibitory activity on the IL-2 and IFN-gamma production of T cells probably via inhibition of the MAPK pathway. The findings will contribute to the development of efficient biocatalysts to create bioactive macrocyclic sesterterpenoids, and also herald a new potential in the well-trodden territory of plant terpenoid biosynthesis. |
| WOS记录号 | WOS:000711812800001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/73451]  |
| 专题 | 中国科学院昆明植物研究所 |
| 作者单位 | 1.Max Planck Inst Chem Ecol, D-07745 Jena, Germany 2.Chengdu Univ Tradit Chinese Med, State Key Lab Southwestern Chinese Med Resources, Chengdu 611137, Peoples R China 3.Chengdu Univ Tradit Chinese Med, Innovat Inst Chinese Med & Pharm, Chengdu 611137, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 5.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Nat Med Chem, Kunming 650201, Yunnan, Peoples R China 6.Wuyi Univ, Sch Biotechnol & Hlth Sci, Jiangmen 529020, Peoples R China 7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen,Yue-Gui,Li,De-Sen,Ling,Yi,et al. A Cryptic Plant Terpene Cyclase Producing Unconventional 18-and 14-Membered Macrocyclic C-25 and C-20 Terpenoids with Immunosuppressive Activity[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2021,60(48):25468-25476. |
| APA | Chen,Yue-Gui.,Li,De-Sen.,Ling,Yi.,Liu,Yan-Chun.,Zuo,Zhi-Li.,...&Li,Sheng-Hong.(2021).A Cryptic Plant Terpene Cyclase Producing Unconventional 18-and 14-Membered Macrocyclic C-25 and C-20 Terpenoids with Immunosuppressive Activity.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,60(48),25468-25476. |
| MLA | Chen,Yue-Gui,et al."A Cryptic Plant Terpene Cyclase Producing Unconventional 18-and 14-Membered Macrocyclic C-25 and C-20 Terpenoids with Immunosuppressive Activity".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 60.48(2021):25468-25476. |
入库方式： OAI收割
来源：昆明植物研究所
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