Biochemical and Metabolic Insights into Hyoscyamine Dehydrogenase
文献类型：期刊论文
| 作者 | Qiu,Fei; Yan,Yijun; Zeng,Junlan; Huang,Jian-Ping; Zeng,Lingjiang; Zhong,Wei; Lan,Xiaozhong; Chen,Min; Huang,Sheng-Xiong; Liao,Zhihua |
| 刊名 | ACS CATALYSIS
 |
| 出版日期 | 2021 |
| 卷号 | 11期号:5页码:2912-2924 |
| 关键词 | biosynthesis crystal structure hyoscyamine hyoscyamine dehydrogenase metabolic engineering TROPANE ALKALOID BIOSYNTHESIS TRANSGENIC ATROPA-BELLADONNA POLYKETIDE SYNTHASE ROOT CULTURES SCOPOLAMINE PUTRESCINE PLANTS 6-BETA-HYDROXYLASE CYTOCHROME-P450 PATHWAY |
| ISSN号 | 2155-5435 |
| DOI | 10.1021/acscatal.0c04667 |
| 英文摘要 | Hyoscyamine, a member of the class of compounds known as tropane alkaloids (TAs), is clinically used as an anticholinergic drug. Previous research has predicted that hyoscyamine is produced via the reduction of hyoscyamine aldehyde. In this study, we identified a root-expressed gene from Atropa belladonna, named AbHDH, which encodes a hyoscyamine dehydrogenase involved in the formation of hyoscyamine. Enzymatic assays indicated that AbHDH was able to not only reduce hyoscyamine aldehyde to produce hyoscyamine but also oxidize hyoscyamine to form hyoscyamine aldehyde under different conditions. To elucidate its catalytic mechanism, the crystal structure of AbHDH at a 2.4-A resolution was also determined. Overexpression of AbHDH significantly enhanced the biosynthesis of hyoscyamine and the production of anisodamine and scopolamine in root cultures of A. belladonna. However, suppression of AbHDH using RNAi technology did not reduce the production of hyoscyamine, anisodamine, or scopolamine, though it did increase the accumulation of hyoscyamine aldehyde. In summary, this study provided timely biochemical and metabolic insights into hyoscyamine dehydrogenase, pointing to an alternative, promising way to produce pharmaceutical TAs via metabolic engineering in planta. |
| WOS记录号 | WOS:000626844200041 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/73483]  |
| 专题 | 中国科学院昆明植物研究所 |
| 作者单位 | 1.Xizang Agr & Anim Husb Coll, TAAHC SWU Med Plant Joint R&D Ctr, Tibetan Collaborat Innovat Ctr Agr & Anim Husb Re, Nyingchi Of Tibet 860000, Peoples R China 2.Southwest Univ, Minist Educ, Key Lab Luminescent & Real Time Analyt Chem, Coll Pharmaceut Sci, Chongqing 400715, Peoples R China 3.Southwest Univ, SWU TAAHC Med Plant Joint R&D Ctr, Chongqing Key Lab Plant Resource Conservat & Germ, Sch Life Sci,Minist Educ,Key Lab Ecoenvironm Thre, Chongqing 400715, Peoples R China 4.Chinese Acad Sci, State Key Lab Phytochem & Plant Resources West Ch, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 5.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiu,Fei,Yan,Yijun,Zeng,Junlan,et al. Biochemical and Metabolic Insights into Hyoscyamine Dehydrogenase[J]. ACS CATALYSIS,2021,11(5):2912-2924. |
| APA | Qiu,Fei.,Yan,Yijun.,Zeng,Junlan.,Huang,Jian-Ping.,Zeng,Lingjiang.,...&Liao,Zhihua.(2021).Biochemical and Metabolic Insights into Hyoscyamine Dehydrogenase.ACS CATALYSIS,11(5),2912-2924. |
| MLA | Qiu,Fei,et al."Biochemical and Metabolic Insights into Hyoscyamine Dehydrogenase".ACS CATALYSIS 11.5(2021):2912-2924. |
入库方式： OAI收割
来源：昆明植物研究所
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