Combined mass spectrometry-guided genome mining and virtual screening for acaricidal activity in secondary metabolites of Bacillus velezensis W1
文献类型:期刊论文
作者 | Li,Xingyu; Munir,Shahzad; Xu,Yan; Wang,Yuehu; He,Yueqiu |
刊名 | RSC ADVANCES |
出版日期 | 2021 |
卷号 | 11期号:41页码:25441-25449 |
ISSN号 | 2046-2069 |
关键词 | BACILLOMYCIN |
DOI | 10.1039/d1ra01326b |
英文摘要 | A comprehensive analytic strategy was performed to study the acaricidal activity ingredients of Bacillus velezensis W1, a strain for biological control of Tetranychus urticae. Through genome mining, 14 biosynthetic gene clusters were identified, which encode secondary metabolites, and these were further confirmed by MALDI-TOF-MS or LC-ESI-MS/MS, including bacillomycin D C13-C17, macrolactin A, 7-O-malonyl-macrolactin A, surfactin C14, and surfactin C15. Moreover, 27 volatile compounds were identified by GC-MS, mainly including cyclodipeptides, alkanes, organic acids, and esters. Finally, 43 compounds identified from W1 were used in the virtual screening of acaricidal activity. The results showed that 16 compounds, including cyclodipeptides, bacillomycins, macrolactins, and surfactins, have acaricidal potential. This work provides a base for studying the mechanism of acaricidal action of B. velezensis W1 and a comprehensive strategy for the study of active ingredients from biocontrol strains. |
WOS记录号 | WOS:000678294800029 |
源URL | [http://ir.kib.ac.cn/handle/151853/73497] |
专题 | 中国科学院昆明植物研究所 |
作者单位 | 1.Yunnan Agr Univ, Coll Sci, Kunming 650201, Yunnan, Peoples R China 2.Yunnan Agr Univ, State Key Lab Conservat & Utilizat Bioresources, Kunming 650201, Yunnan, Peoples R China 3.Cleveland State Univ, Dept Chem, Cleveland, OH 44115 USA 4.Chinese Acad Sci, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China |
推荐引用方式 GB/T 7714 | Li,Xingyu,Munir,Shahzad,Xu,Yan,et al. Combined mass spectrometry-guided genome mining and virtual screening for acaricidal activity in secondary metabolites of Bacillus velezensis W1[J]. RSC ADVANCES,2021,11(41):25441-25449. |
APA | Li,Xingyu,Munir,Shahzad,Xu,Yan,Wang,Yuehu,&He,Yueqiu.(2021).Combined mass spectrometry-guided genome mining and virtual screening for acaricidal activity in secondary metabolites of Bacillus velezensis W1.RSC ADVANCES,11(41),25441-25449. |
MLA | Li,Xingyu,et al."Combined mass spectrometry-guided genome mining and virtual screening for acaricidal activity in secondary metabolites of Bacillus velezensis W1".RSC ADVANCES 11.41(2021):25441-25449. |
入库方式: OAI收割
来源:昆明植物研究所
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