Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities
文献类型：期刊论文
| 作者 | Ma,Yan; Gao,Na; Zuo,Zhichuang; Li,Shanni; Zheng,Wenqi; Shi,Xiang; Liu,Qipei; Ma,Ting; Yin,Ronghua; Li,Xian |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2021 |
| 卷号 | 186页码:535-543 |
| ISSN号 | 0141-8130 |
| 关键词 | Sea cucumber Fucan sulfate Chemical structure Anticoagulant Pattalus mollis FUCOSYLATED GLYCOSAMINOGLYCAN REPEATING UNIT FUCOIDAN POLYSACCHARIDES ELUCIDATION OLIGOSACCHARIDES SPECTROSCOPY |
| DOI | 10.1016/j.ijbiomac.2021.07.049 |
| 英文摘要 | Fucan sulfates from echinoderm possess characteristic structures and various biological activities. Herein, comprehensive methods including enzymolysis, ion-exchange chromatography and size exclusion chromatography lead to the purification of five fucan sulfates (FSI, FSII, FSIII, FSIV, FSV) from the sea cucumber Pattalus mollis. Chemical composition analysis showed that they were all composed of L-fucose. Their sulfate content was determined by a conductimetric method. The molecular weight (Mw) of FSI, FSII, FSIII, FSIV and FSV were measured as 238.3 kDa, 81.0 kDa, 82.0 kDa, 23.2 kDa and 6.12 kDa, respectively. Detailed NMR spectroscopic analysis revealed that the structural sequence of FSI and FSII was -> 3)-L-Fuc(S)-alpha(1 ->, where Fuc(S) were Fuc(2S4S) (10%), Fuc(2S) (44%), Fuc(0S) (10%), Fuc(4S) (36%), that of FSIII was -> 4)-L-Fuc(2S)-(alpha 1 -> 4)-L-Fuc(2S)-(alpha 1 -> 4)-L-Fuc(0S/3S)-(alpha 1 -> where Fuc(0S) and Fuc(3S) were in equal molar, and that FSIV was -> 4)-L-Fuc(2S3S)-(alpha 1 -> 4)-L-Fuc(2S3S)-(alpha 1 -> 4)-L-Fuc(2S)-(alpha 1 -> 4)-L-Fuc(2S)-(alpha 1 -> 4)-L-Fuc(2S)-(alpha 1 ->. This is the first report that such a diversity of fucan sulfates were obtained from the same sea cucumber species. Biological activity showed that FSI, FSII, FSIII and FSIV exhibited potent anticoagulant by prolonging the APTT. Among them, FSII, FSIII and FSIV showed the similar potency, while FSI owned the strongest. Structure-activity relationships analysis showed that molecular weight and sulfation degree should be the crucial factors for the activity. |
| WOS记录号 | WOS:000688434600010 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/73500]  |
| 专题 | 中国科学院昆明植物研究所 |
| 作者单位 | 1.South Cent Univ Natl, Sch Pharmaceut Sci, Wuhan 430074, Peoples R China 2.Kunming Med Univ, Sch Pharmaceut Sci, Kunming 650500, Yunnan, Peoples R China 3.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming 650500, Yunnan, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma,Yan,Gao,Na,Zuo,Zhichuang,et al. Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,186:535-543. |
| APA | Ma,Yan.,Gao,Na.,Zuo,Zhichuang.,Li,Shanni.,Zheng,Wenqi.,...&Zhao,Jinhua.(2021).Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,186,535-543. |
| MLA | Ma,Yan,et al."Five distinct fucan sulfates from sea cucumber Pattalus mollis: Purification, structural characterization and anticoagulant activities".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 186(2021):535-543. |
入库方式： OAI收割
来源：昆明植物研究所
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