Lipid Remodeling Confers Osmotic Stress Tolerance to Embryogenic Cells during Cryopreservation
文献类型：期刊论文
| 作者 | Lin,Liang; Ma,Junchao; Ai,Qin; Pritchard,Hugh W.; Li,Weiqi ; Chen,Hongying
|
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2021 |
| 卷号 | 22期号:4页码:2174 |
| 关键词 | cryopreservation cryoprotectant agents ex situ conservation lipid Magnoliaceae plant vitrification solutions |
| ISSN号 | 1422-0067 |
| DOI | 10.3390/ijms22042174 |
| 英文摘要 | Plant species conservation through cryopreservation using plant vitrification solutions (PVS) is based in empiricism and the mechanisms that confer cell integrity are not well understood. Using ESI-MS/MS analysis and quantification, we generated 12 comparative lipidomics datasets for membranes of embryogenic cells (ECs) of Magnolia officinalis during cryogenic treatments. Each step of the complex PVS-based cryoprotocol had a profoundly different impact on membrane lipid composition. Loading treatment (osmoprotection) remodeled the cell membrane by lipid turnover, between increased phosphatidic acid (PA) and phosphatidylglycerol (PG) and decreased phosphatidylcholine (PC) and phosphatidylethanolamine (PE). The PA increase likely serves as an intermediate for adjustments in lipid metabolism to desiccation stress. Following PVS treatment, lipid levels increased, including PC and PE, and this effectively counteracted the potential for massive loss of lipid species when cryopreservation was implemented in the absence of cryoprotection. The present detailed cryobiotechnology findings suggest that the remodeling of membrane lipids and attenuation of lipid degradation are critical for the successful use of PVS. As lipid metabolism and composition varies with species, these new insights provide a framework for technology development for the preservation of other species at increasing risk of extinction. |
| WOS记录号 | WOS:000623935100001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/73505]  |
| 专题 | 中国科学院昆明植物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Yunnan, Peoples R China 2.[Lin, Liang 3.Ma, Junchao 4.Ai, Qin 5.Pritchard, Hugh W. 6.Li, Weiqi 7.[Pritchard, Hugh W.] Royal Bot Gardens, Wellcome Trust Millennium Bldg,Wakehurst Pl, Ardingly RH17 6TN, W Sussex, England |
| 推荐引用方式 GB/T 7714 | Lin,Liang,Ma,Junchao,Ai,Qin,et al. Lipid Remodeling Confers Osmotic Stress Tolerance to Embryogenic Cells during Cryopreservation[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(4):2174. |
| APA | Lin,Liang,Ma,Junchao,Ai,Qin,Pritchard,Hugh W.,Li,Weiqi,&Chen,Hongying.(2021).Lipid Remodeling Confers Osmotic Stress Tolerance to Embryogenic Cells during Cryopreservation.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(4),2174. |
| MLA | Lin,Liang,et al."Lipid Remodeling Confers Osmotic Stress Tolerance to Embryogenic Cells during Cryopreservation".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.4(2021):2174. |
入库方式： OAI收割
来源：昆明植物研究所
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