Side Products of Recombinant Amorpha-4,11-diene Synthase and Their Effect on Microbial Artemisinin Production
文献类型：期刊论文
| 作者 | Huang,Jin-Quan; Li,Dong-Mei; Tian,Xiu; Lin,Jia-Ling; Yang,Lei; Xu,Jing-Jing; Fang,Xin |
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| 出版日期 | 2021 |
| 卷号 | 69期号:7页码:2168-2178 |
| 关键词 | artemisinin chiral analysis cytochrome P450 monooxygenases labeling sesquiterpenes specialized metabolism pathway terpene synthases |
| ISSN号 | 0021-8561 |
| DOI | 10.1021/acs.jafc.0c07462 |
| 英文摘要 | Amorpha-4,11-diene synthase (ADS) is the first committed enzyme in the biosynthesis of artemisinin. Artemisinin production by biobased fermentation is considered a reliable alternative pathway. Heterologously expressed ADS has been established to generate several minor products, including structural analogues of amorpha-4,11-diene, but their fate in fermentation is still unknown. Here, using chiral analysis, we found that ADS produces one of the analogues, amorpha-4-en-11-ol, as a pair of epimers. Labeling experiments revealed that ADS mutants yielded amorphene-type sesquiterpenes, indicating the co-occurrence of initial 1,6 and 1,10 cyclization of farnesyl diphosphate in a single enzyme. Interestingly, the immediate downstream oxidase CYP71AV1 had very low affinity to the side products of the recombinant ADS, including amorpha-4-en-7-ol, which is structurally similar to amorpha-4,11-diene. Our data uncover the complex catalytic mechanism of recombinant ADS and reveal a potential negative effect of the side products of recombinant ADS on the production of the artemisinin precursor in microbes. |
| WOS记录号 | WOS:000624326000015 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/73698]  |
| 专题 | 中国科学院昆明植物研究所 |
| 作者单位 | 1.[Huang, Jin-Quan 2.Yunnan Univ, Kunming 650091, Yunnan, Peoples R China 3.Tian, Xiu 4.Chinese Acad Sci, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, CAS Ctr Excellence Mol Plant Sci, Shanghai 200032, Peoples R China 5.[Li, Dong-Mei 6.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650204, Yunnan, Peoples R China 7.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 200031, Peoples R China 8.[Yang, Lei 9.Shanghai Chenshan Bot Garden, Shanghai Key Lab Plant Funct Genom & Resources, Plant Sci Res Ctr, Shanghai 201602, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang,Jin-Quan,Li,Dong-Mei,Tian,Xiu,et al. Side Products of Recombinant Amorpha-4,11-diene Synthase and Their Effect on Microbial Artemisinin Production[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2021,69(7):2168-2178. |
| APA | Huang,Jin-Quan.,Li,Dong-Mei.,Tian,Xiu.,Lin,Jia-Ling.,Yang,Lei.,...&Fang,Xin.(2021).Side Products of Recombinant Amorpha-4,11-diene Synthase and Their Effect on Microbial Artemisinin Production.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,69(7),2168-2178. |
| MLA | Huang,Jin-Quan,et al."Side Products of Recombinant Amorpha-4,11-diene Synthase and Their Effect on Microbial Artemisinin Production".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 69.7(2021):2168-2178. |
入库方式： OAI收割
来源：昆明植物研究所
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