The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice
文献类型：期刊论文
| 作者 | Hu,Jihong; Huang,Liyu; Chen,Guanglong; Liu,Hui; Zhang,Yesheng; Zhang,Ru; Zhang,Shilai; Liu,Jintao; Hu,Qingyi; Hu,Fengyi |
| 刊名 | RICE
 |
| 出版日期 | 2021 |
| 卷号 | 14期号:1页码:90 |
| 关键词 | OsSPL4 Grain size Grain weight Panicle branching Grain yield Rice NATURAL VARIATION SBP-DOMAIN RARE ALLELE GENE BINDING IDENTIFICATION EXPRESSION PROTEIN DISSECTION PATHWAYS |
| ISSN号 | 1939-8425 |
| DOI | 10.1186/s12284-021-00531-7 |
| 英文摘要 | Grain weight and grain number, the two important yield traits, are mainly determined by grain size and panicle architecture in rice. Herein, we report the identification and functional analysis of OsSPL4 in panicle and grain development of rice. Using CRISPR/Cas9 system, two elite alleles of OsSPL4 were obtained, which exhibited an increasing number of grains per panicle and grain size, resulting in increase of rice yield. Cytological analysis showed that OsSPL4 could regulate spikelet development by promoting cell division. The results of RNA-seq and qRT-PCR validations also demonstrated that several MADS-box and cell-cycle genes were up-regulated in the mutation lines. Co-expression network revealed that many yield-related genes were involved in the regulation network of OsSPL4. In addition, OsSPL4 could be cleaved by the osa-miR156 in vivo, and the OsmiR156-OsSPL4 module might regulate the grain size in rice. Further analysis indicated that the large-grain allele of OsSPL4 in indica rice might introgress from aus varieties under artificial selection. Taken together, our findings suggested that OsSPL4 could be as a key regulator of grain size by acting on cell division control and provided a strategy for panicle architecture and grain size modification for yield improvement in rice. |
| WOS记录号 | WOS:000714027400001 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/73718]  |
| 专题 | 中国科学院昆明植物研究所 |
| 作者单位 | 1.BGI Baoshan, Baoshan 678004, Yunnan, Peoples R China 2.Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan 430072, Peoples R China 3.Chinese Acad Sci, Kunming Inst Zool, State Key Lab Genet Resources & Evolut, Kunming 650223, Yunnan, Peoples R China 4.Northwestern Polytech Univ, Sch Ecol & Environm, Xian 710072, Peoples R China 5.Yunnan Univ, Sch Agr, State Key Lab Conservat & Utilizat Bioresources Y, Kunming 650500, Yunnan, Peoples R China 6.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu,Jihong,Huang,Liyu,Chen,Guanglong,et al. The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice[J]. RICE,2021,14(1):90. |
| APA | Hu,Jihong.,Huang,Liyu.,Chen,Guanglong.,Liu,Hui.,Zhang,Yesheng.,...&Ding,Yi.(2021).The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice.RICE,14(1),90. |
| MLA | Hu,Jihong,et al."The Elite Alleles of OsSPL4 Regulate Grain Size and Increase Grain Yield in Rice".RICE 14.1(2021):90. |
入库方式： OAI收割
来源：昆明植物研究所
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