Structural characterization and anticoagulant analysis of the novel branched fucosylated glycosaminoglycan from sea cucumber Holothuria nobilis
文献类型：期刊论文
| 作者 | Li,Shanni; Zhong,Wei; Pan,Ying; Lin,Lisha; Cai,Ying; Mao,Hui; Zhang,Taocui; Li,Sujuan; Chen,Ru; Zhou,Lutan |
| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2021 |
| 卷号 | 269页码:118290 |
| ISSN号 | 0144-8617 |
| 关键词 | Disaccharide branch Fucosylated glycosaminoglycan Holothuria nobilis Oligosaccharide Anticoagulant INTRINSIC TENASE COMPLEX CHONDROITIN SULFATE ACAUDINA-MOLPADIOIDEA FACTOR XASE HEPARIN DEPOLYMERIZATION OLIGOSACCHARIDES POLYSACCHARIDES DERIVATIVES |
| DOI | 10.1016/j.carbpol.2021.118290 |
| 英文摘要 | Glycosaminoglycan HnFG was extracted from sea cucumber Holothuria nobilis. Its chemical structure was characterized by analyzing the physicochemical properties, oligosaccharides from its mild acid hydrolysates and depolymerized products. The disaccharide D-GalNAc4S6S-alpha 1,2-L-Fuc3S-ol found in its mild acid hydrolysates provided a clue for the presence of a unique disaccharide-branch in HnFG. Furthermore, it was confirmed by a series of oligosaccharides from the low-molecular weight HnFG prepared by beta-eliminative depolymerization. Combining with the analysis of its peroxide depolymerized products, the precise structure of HnFG was determined: A chondroitin sulfate E (CS-E)-like backbone branched with sulfated monofucoses (-67%) and disaccharides D-GalNAcS-alpha 1,2-L-Fuc3S (-33%) at O-3 position of each GlcUA. This is the first report on the novel branches in glycosaminoglycan. Biologically, the native and depolymerized HnFG showed potent activities in prolonging the activated partial thrombin time (APTT) and inhibiting intrinsic coagulation Xase (iXase), whereas the oligosaccharides (degree of polymerization <= 6) had no obvious effects. |
| WOS记录号 | WOS:000678038500006 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/73735]  |
| 专题 | 中国科学院昆明植物研究所 |
| 作者单位 | 1.Guangxi Inst Tradit Med & Pharmaceut Sci, Nanning 530022, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 3.South Cent Univ Nat, Sch Pharmaceut Sci, Wuhan 430074, Peoples R China 4.Yunnan Univ, Sch Life Sci, Kunming 650500, Yunnan, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li,Shanni,Zhong,Wei,Pan,Ying,et al. Structural characterization and anticoagulant analysis of the novel branched fucosylated glycosaminoglycan from sea cucumber Holothuria nobilis[J]. CARBOHYDRATE POLYMERS,2021,269:118290. |
| APA | Li,Shanni.,Zhong,Wei.,Pan,Ying.,Lin,Lisha.,Cai,Ying.,...&Zhao,Jinhua.(2021).Structural characterization and anticoagulant analysis of the novel branched fucosylated glycosaminoglycan from sea cucumber Holothuria nobilis.CARBOHYDRATE POLYMERS,269,118290. |
| MLA | Li,Shanni,et al."Structural characterization and anticoagulant analysis of the novel branched fucosylated glycosaminoglycan from sea cucumber Holothuria nobilis".CARBOHYDRATE POLYMERS 269(2021):118290. |
入库方式： OAI收割
来源：昆明植物研究所
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