Green synthesis of broccoli-derived carbon quantum dots as effective photosensitizers for the PDT effect testified in the model of mutant Caenorhabditis elegans
文献类型：期刊论文
| 作者 | Elsherbiny, Shereen M.2,3,4; Shao, Changsheng3,4; Acheampong, Adolf3,4; Khalifa, Mahmoud A.1,2; Liu, Chao3,4; Huang, Qing3,4
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| 刊名 | BIOMATERIALS SCIENCE
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| 出版日期 | 2022-04-05 |
| ISSN号 | 2047-4830 |
| DOI | 10.1039/d2bm00274d |
| 通讯作者 | Huang, Qing(huangq@ipp.ac.cn) |
| 英文摘要 | The clinical application of photodynamic therapy (PDT) is still limited because of the drawbacks of the traditional photosensitizers, such as low singlet oxygen (O-1(2)) quantum yield and the problem of photobleaching. Herein, carbon quantum dots (CQDs) derived from broccoli natural biomass as a carbon source were fabricated via a simple hydrothermal method and showed outstanding PDT ability as an effective photodynamic agent tested in Caenorhabditis elegans (C. elegans) models. The as-prepared broccoli-derived CQDs (BCQDs) showed excellent water solubility and optical properties and could generate singlet oxygen (O-1(2)) effectively under irradiated light with a wavelength of 660 nm. The in vivo experiment revealed that the PDT efficiency of the BCQDs was dependent on the induction of germline apoptosis through the cep-1/p53 pathway. Further investigation confirmed the DNA damage of the worm by the BCQDs after sufficient light irradiation, which was tested by measuring the egl-1-fold induction in hus-1(op244), and cep-1(w40) mutants that have a loss of function in the genes involved in DNA damage response such as hus-1 (DNA checkpoint gene) and cep-1/p53 (tumor suppressor). The lack of germline apoptosis in the loss of function mutants egl-1(n487), hus-1(op244), and cep-1(w40) exposed to light irradiation compared with the control proved the necessity of these genes in DNA damage-induced germline apoptosis. Therefore, this work has not only provided a new photodynamic agent but also introduced C. elegans as an easy and high-throughput model for the rapid evaluation of the efficiency of PDT. |
| 资助项目 | National Natural Science Foundation of China[11635013] ; CAS-TWAS President's Fellowship |
| WOS研究方向 | Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000785865300001 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 资助机构 | National Natural Science Foundation of China ; CAS-TWAS President's Fellowship |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/128469]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Huang, Qing |
| 作者单位 | 1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Peoples R China 2.Mansoura Univ, Fac Sci, Dept Phys, Mansoura 35516, Egypt 3.Univ Sci & Technol China, Grad Sch, Sci Isl Branch, Hefei 230026, Peoples R China 4.Chinese Acad Sci, Inst Intelligent Machine, CAS Key Lab High Magnet Field & Ion Beam Phys Bio, Hefei Inst Phys Sci,Anhui Key Lab Environm Toxico, Hefei 230031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Elsherbiny, Shereen M.,Shao, Changsheng,Acheampong, Adolf,et al. Green synthesis of broccoli-derived carbon quantum dots as effective photosensitizers for the PDT effect testified in the model of mutant Caenorhabditis elegans[J]. BIOMATERIALS SCIENCE,2022. |
| APA | Elsherbiny, Shereen M.,Shao, Changsheng,Acheampong, Adolf,Khalifa, Mahmoud A.,Liu, Chao,&Huang, Qing.(2022).Green synthesis of broccoli-derived carbon quantum dots as effective photosensitizers for the PDT effect testified in the model of mutant Caenorhabditis elegans.BIOMATERIALS SCIENCE. |
| MLA | Elsherbiny, Shereen M.,et al."Green synthesis of broccoli-derived carbon quantum dots as effective photosensitizers for the PDT effect testified in the model of mutant Caenorhabditis elegans".BIOMATERIALS SCIENCE (2022). |
入库方式： OAI收割
来源：合肥物质科学研究院
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