中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 合肥物质科学研究院
  3. 中国科学院合肥物质科学研究院
Rapid One-Tube RPA-CRISPR/Cas12 Detection Platform for Methicillin-Resistant Staphylococcus aureus

文献类型：期刊论文

作者Li, Yanan2,3; Shi, Zhonglin1,3; Hu, Anzhong3; Cui, Junsheng3; Yang, Ke3; Liu, Yong3; Deng, Guoqing3; Zhu, Cancan3; Zhu, Ling3
刊名DIAGNOSTICS
出版日期2022-04-01
卷号12
关键词methicillin-resistant Staphylococcus aureus mecA gene RPA-CRISPR/Cas12a one-tube POCT
DOI10.3390/diagnostics12040829
通讯作者Zhu, Cancan(zhucancan@aiofm.ac.cn) ; Zhu, Ling(zhul@aiofm.ac.cn)
英文摘要Methicillin-resistant Staphylococcus aureus (MRSA) is a severe health threat causing highlevel morbidity and mortality in health care environments and in community settings. Though existing diagnostic methods, including PCR and culture-based methods, are routinely used in clinical practice, they are not appropriate for rapid point-of-care testing (POCT). Recently, since the development of the CRISPR/Cas technology, new possibilities for rapid point-of-care detection have emerged. In this study, we developed a rapid, accurate, and contamination-free platform for MRSA detection by integrating recombinase polymerase amplification (RPA) with the Cas12 system into one tube. Using this approach, visual MRSA detection could be achieved in 20 min. Based on the one-tube RPA-CRISPR/Cas12a platform, the assay results are visualized by lateral flow test strips (LFS) and fluorescent-based methods, including real-time and end-point fluorescence. This platform allows specific MRSA detection with a sensitivity of 10 copies for the fluorescence method and a range of 10-100 copies for the LFS. The results of 23 samples from clinical MRSA isolates showed that the coincidence rate was 100% and 95.7% of the fluorescence method and LFS, respectively, compared to qPCR. In conclusion, the one-tube RPA-CRISPR/Cas12a platform is an effective method for MRSA detection with significant potential in future practical POCT applications.
WOS关键词NUCLEIC-ACID DETECTION ; EPIDEMIOLOGY ; INFECTIONS
资助项目National Natural Science Foundation of China[82002189] ; Natural Science Foundation of Anhui Province of China[2008085QF311] ; Key Research and Development Program of Anhui Province of China[202004d07020014] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2018489] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2021442] ; Dean's Fund of Hefei Institute of Physical Science, China[YZJJ2020QN35] ; Dean's Fund of Hefei Institute of Physical Science, China[YZJJ2021QN03]
WOS研究方向General & Internal Medicine
语种英语
出版者MDPI
WOS记录号WOS:000786055700001
资助机构National Natural Science Foundation of China ; Natural Science Foundation of Anhui Province of China ; Key Research and Development Program of Anhui Province of China ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; Dean's Fund of Hefei Institute of Physical Science, China
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/128537]  
专题中国科学院合肥物质科学研究院
通讯作者Zhu, Cancan; Zhu, Ling
作者单位1.Anhui Univ, Inst Phys Sci & Informat Technol, Hefei 230601, Peoples R China
2.Univ Sci & Technol China, Sci Isl Branch, Grad Sch, Hefei 230026, Peoples R China
3.Chinese Acad Sci, Hefei Inst Phys Sci, Hefei 230031, Peoples R China
推荐引用方式
GB/T 7714		 Li, Yanan,Shi, Zhonglin,Hu, Anzhong,et al. Rapid One-Tube RPA-CRISPR/Cas12 Detection Platform for Methicillin-Resistant Staphylococcus aureus[J]. DIAGNOSTICS,2022,12.
APA Li, Yanan.,Shi, Zhonglin.,Hu, Anzhong.,Cui, Junsheng.,Yang, Ke.,...&Zhu, Ling.(2022).Rapid One-Tube RPA-CRISPR/Cas12 Detection Platform for Methicillin-Resistant Staphylococcus aureus.DIAGNOSTICS,12.
MLA Li, Yanan,et al."Rapid One-Tube RPA-CRISPR/Cas12 Detection Platform for Methicillin-Resistant Staphylococcus aureus".DIAGNOSTICS 12(2022).

入库方式： OAI收割

来源：合肥物质科学研究院

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。