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A Peptide from Budding Yeast GAPDH Serves as a Promising Antifungal against Cryptococcus neoformans

文献类型：期刊论文

作者Zhang, Yang5,6; Zhou, Liyan1,4,5; Liu, Yan4,5; Zhao, Xi4,5; Lian, Xianqiang4,5; Zhang, Jie5; Zhao, De4,5; Wang, Yujuan3; Zhong, Jin5; Wang, Junfeng3
刊名MICROBIOLOGY SPECTRUM
出版日期2022-02-01
卷号10
关键词antifungal peptide GAPDH Cryptococcus neoformans apoptosis
ISSN号2165-0497
DOI10.1128/spectrum.00826-21
通讯作者Zhou, Liyan() ; Wang, Linqi(wanglq@im.ac.cn) ; Fu, Yu, V(fuyu@im.ac.cn)
英文摘要Infection of Cryptococcus neoformans is one of the leading causes of morbidity and mortality, particularly among immunocompromised patients. However, currently available drugs for the treatment of C. neoformans infection are minimal. Here, we report SP1, a peptide derived from glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Saccharomyces cerevisiae, efficiently kills C. neoformans and Cryptococcus gattii. SP1 causes damages to the capsule. Unlike many antimicrobial peptides, SP1 does not form pores on the cell membrane of C. neoformans. It interacts with membrane ergosterol and enters vacuole possibly through membrane trafficking. C. neoformans treated with SP1 show the apoptotic phenotypes such as imbalance of calcium ion homeostasis, reactive oxygen increment, phosphatidylserine exposure, and nuclear fragmentation. Our data imply that SP1 has the potential to be developed into a treatment option for cryptococcosis. IMPORTANCE Cryptococcus neoformans and Cryptococcus gattii can cause cryptococcosis, which has a high mortality rate. To treat the disease, amphotericin B and fluconazole are often used in clinic. However, amphotericin B has rather high renal toxicity, and tolerance to these drugs are quicky developed. The peptide SP1 derived from baker's yeast GAPDH shows antifungal function to kill Cryptococcus neoformans and Cryptococcus gattii efficiently with a high specificity, even for the drug-resistant strains. Our data demonstrate that SP1 induces the apoptosis-like death of Cryptococcus neoformans at low concentrations. The finding of this peptide may shed light on a new direction to treat cryptococcosis.
WOS关键词PROTEIN SECONDARY STRUCTURE ; CIRCULAR-DICHROISM SPECTROSCOPY ; ANTIMICROBIAL PEPTIDES ; SWISS-MODEL ; HIGH POTENCY ; MEMBRANE ; DIVERSITY ; VIRULENCE ; CA2+
资助项目National Natural Science Foundation of China[31970553] ; National Natural Science Foundation of China[31571288] ; National Natural Science Foundation of China[52091541] ; Chinese Academy of Sciences[GQRC-19-18] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA17010503] ; Senior User Project of RV KEXUE[KEXUE2019GZ05] ; Royal Society[NA140085]
WOS研究方向Microbiology
语种英语
WOS记录号WOS:000766015800024
出版者AMER SOC MICROBIOLOGY
资助机构National Natural Science Foundation of China ; Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Senior User Project of RV KEXUE ; Royal Society
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/128667]  
专题中国科学院合肥物质科学研究院
通讯作者Zhou, Liyan; Wang, Linqi; Fu, Yu, V
作者单位1.Guangxi Med Univ, Dept Clin Lab, Affiliated Hosp 2, Nanning, Peoples R China
2.Capital Med Univ, Beijing Obstet & Gynecol Hosp, Dept Lab Med, Beijing, Peoples R China
3.Chinese Acad Sci, Hefei Inst Phys Sci, High Magnet Field Lab, Hefei, Peoples R China
4.Univ Chinese Acad Sci, Savaid Med Sch, Beijing, Peoples R China
5.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing, Peoples R China
6.Univ Sci & Technol China, Sch Life Sci, Div Life Sci & Med, Hefei, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Yang,Zhou, Liyan,Liu, Yan,et al. A Peptide from Budding Yeast GAPDH Serves as a Promising Antifungal against Cryptococcus neoformans[J]. MICROBIOLOGY SPECTRUM,2022,10.
APA Zhang, Yang.,Zhou, Liyan.,Liu, Yan.,Zhao, Xi.,Lian, Xianqiang.,...&Fu, Yu, V.(2022).A Peptide from Budding Yeast GAPDH Serves as a Promising Antifungal against Cryptococcus neoformans.MICROBIOLOGY SPECTRUM,10.
MLA Zhang, Yang,et al."A Peptide from Budding Yeast GAPDH Serves as a Promising Antifungal against Cryptococcus neoformans".MICROBIOLOGY SPECTRUM 10(2022).

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来源：合肥物质科学研究院

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