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Vitamin K2 Modulates Mitochondrial Dysfunction Induced by 6-Hydroxydopamine in SH-SY5Y Cells via Mitochondrial Quality-Control Loop

文献类型：期刊论文

作者Tang, Hengfang3,4; Zheng, Zhiming4; Wang, Han4; Wang, Li4; Zhao, Genhai4; Wang, Peng1,2
刊名NUTRIENTS
出版日期2022-04-01
卷号14
关键词vitamin K2 apoptosis ROS mitochondrial dysfunction mitophagy mitochondrial biogenesis mitochondrial quality-control loop PINK1 Parkin signaling pathway
DOI10.3390/nu14071504
通讯作者Zheng, Zhiming(zhengzhiming2014@163.com) ; Wang, Peng(pengwang@ipp.ac.cn)
英文摘要Vitamin K2, a natural fat-soluble vitamin, is a potent neuroprotective molecule, owing to its antioxidant effect, but its mechanism has not been fully elucidated. Therefore, we stimulated SH-SY5Y cells with 6-hydroxydopamine (6-OHDA) in a proper dose-dependent manner, followed by a treatment of vitamin K2. In the presence of 6-OHDA, cell viability was reduced, the mitochondrial membrane potential was decreased, and the accumulation of reactive oxygen species (ROS) was increased. Moreover, the treatment of 6-OHDA promoted mitochondria-mediated apoptosis and abnormal mitochondrial fission and fusion. However, vitamin K2 significantly suppressed 6-OHDA-induced changes. Vitamin K2 played a significant part in apoptosis by upregulating and downregulating Bcl-2 and Bax protein expressions, respectively, which inhibited mitochondrial depolarization, and ROS accumulation to maintain mitochondrial structure and functional stabilities. Additionally, vitamin K2 significantly inhibited the 6-OHDA-induced downregulation of the MFN1/2 level and upregulation of the DRP1 level, respectively, and this enabled cells to maintain the dynamic balance of mitochondrial fusion and fission. Furthermore, vitamin K2 treatments downregulated the expression level of p62 and upregulated the expression level of LC3A in 6-OHDA-treated cells via the PINK1/Parkin signaling pathway, thereby promoting mitophagy. Moreover, it induced mitochondrial biogenesis in 6-OHDA damaged cells by promoting the expression of PGC-1 alpha, NRF1, and TFAM. These indicated that vitamin K2 can release mitochondrial damage, and that this effect is related to the participation of vitamin K2 in the regulation of the mitochondrial quality-control loop, through the maintenance of the mitochondrial quality-control system, and repair mitochondrial dysfunction, thereby alleviating neuronal cell death mediated by mitochondrial damage.
WOS关键词OXIDATIVE STRESS ; PARKINSONS ; MITOPHAGY ; PINK1 ; MECHANISMS ; AUTOPHAGY ; DYNAMICS ; PROTECTS ; MODELS ; ROLES
资助项目Major Projects of Science and Technology of Anhui Province[202103a06020003] ; China National Key Research and Development Program[2019YFA0904300] ; China National Key Research and Development Program[2019YFA0904304] ; Key Research and Development Plan of Anhui Province[1804b06020342] ; Natural Science Foundation of Anhui Province[1908085MB48] ; Natural Science Foundation of Anhui Province[1908085MB43] ; National Natural Science Foundation of China[32070088]
WOS研究方向Nutrition & Dietetics
语种英语
WOS记录号WOS:000782044500001
出版者MDPI
资助机构Major Projects of Science and Technology of Anhui Province ; China National Key Research and Development Program ; Key Research and Development Plan of Anhui Province ; Natural Science Foundation of Anhui Province ; National Natural Science Foundation of China
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/128683]  
专题中国科学院合肥物质科学研究院
通讯作者Zheng, Zhiming; Wang, Peng
作者单位1.CAS Hefei Inst Technol Innovat Co Ltd, Hefei 230088, Peoples R China
2.Chinese Acad Sci, Hefei Inst Phys Sci, Anhui Key Lab Environm Toxicol & Pollut Control T, Hefei 230031, Peoples R China
3.Univ Sci & Technol China, Sci Isl Branch Grad, Hefei 230026, Peoples R China
4.Chinese Acad Sci, Hefei Inst Phys Sci, Inst Intelligent Machines, Hefei 230031, Peoples R China
推荐引用方式
GB/T 7714		 Tang, Hengfang,Zheng, Zhiming,Wang, Han,et al. Vitamin K2 Modulates Mitochondrial Dysfunction Induced by 6-Hydroxydopamine in SH-SY5Y Cells via Mitochondrial Quality-Control Loop[J]. NUTRIENTS,2022,14.
APA Tang, Hengfang,Zheng, Zhiming,Wang, Han,Wang, Li,Zhao, Genhai,&Wang, Peng.(2022).Vitamin K2 Modulates Mitochondrial Dysfunction Induced by 6-Hydroxydopamine in SH-SY5Y Cells via Mitochondrial Quality-Control Loop.NUTRIENTS,14.
MLA Tang, Hengfang,et al."Vitamin K2 Modulates Mitochondrial Dysfunction Induced by 6-Hydroxydopamine in SH-SY5Y Cells via Mitochondrial Quality-Control Loop".NUTRIENTS 14(2022).

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来源：合肥物质科学研究院

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