Engineering microbial consortia of Elizabethkingia meningoseptica and Escherichia coli strains for the biosynthesis of vitamin K2
文献类型：期刊论文
| 作者 | Yang,Qiang2,3; Zheng,Zhiming3 ; Zhao,Genhai3; Wang,Li3; Wang,Han2,3; Ding,XiuMin2,3; Jiang,Chunxu2,3; Li,Chu1,3; Ma,Guoliang2,3; Wang,Peng3
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| 刊名 | Microbial Cell Factories
 |
| 出版日期 | 2022-03-12 |
| 卷号 | 21 |
| 关键词 | Vitamin K2 Microbial consortia Metabolic engineering Elizabethkingia meningoseptica |
| DOI | 10.1186/s12934-022-01768-7 |
| 通讯作者 | Zheng,Zhiming(zhengzhiming2014@163.com) ; Wang,Peng(pengwang@ipp.ac.cn) |
| 英文摘要 | AbstractBackgroundThe study and application of microbial consortia are topics of interest in the fields of metabolic engineering and synthetic biology. In this study, we report the design and optimisation of Elizabethkingia meningoseptica and Escherichia coli co-culture, which bypass certain limitations found during the molecular modification of E. meningoseptica, such as resistance to many antibiotics and fewer available molecular tools.ResultsThe octaprenyl pyrophosphate synthase from E. meningoseptica sp. F2 (EmOPPS) was expressed, purified, and identified in the present study. Then, owing to the low vitamin K2 production by E. coli or E. meningoseptica sp. F2 monoculture, we introduced the E. meningoseptica and E. coli co-culture strategy to improve vitamin K2 biosynthesis. We achieved production titres of 32?mg/L by introducing vitamin K2 synthesis-related genes from E. meningoseptica sp. F2 into E. coli, which were approximately three-fold more than the titre achieved with E. meningoseptica sp. F2 monoculture. This study establishes a foundation for further engineering of MK-n (n?=?4, 5, 6, 7, 8) in a co-cultivation system of E. meningoseptica and E. coli. Finally, we analysed the surface morphology, esterase activity, and membrane permeability of these microbial consortia using scanning electron microscopy, confocal laser scanning microscopy, and flow cytometry, respectively. The results showed that the co-cultured bacteria were closely linked and that lipase activity and membrane permeability improved, which may be conducive to the exchange of substances between bacteria.ConclusionsOur results demonstrated that co-culture engineering can be a useful method in the broad field of metabolic engineering of strains with restricted molecular modifications. |
| 语种 | 英语 |
| WOS记录号 | BMC:10.1186/S12934-022-01768-7 |
| 出版者 | BioMed Central |
| 源URL | [http://ir.hfcas.ac.cn:8080/handle/334002/128729]  |
| 专题 | 中国科学院合肥物质科学研究院 |
| 通讯作者 | Zheng,Zhiming; Wang,Peng |
| 作者单位 | 1.Hefei Normal University 2.University of Science and Technology of China 3.Chinese Academy of Sciences; Institute of Intelligent Machines, Hefei Institutes of Physical Science |
| 推荐引用方式 GB/T 7714 | Yang,Qiang,Zheng,Zhiming,Zhao,Genhai,et al. Engineering microbial consortia of Elizabethkingia meningoseptica and Escherichia coli strains for the biosynthesis of vitamin K2[J]. Microbial Cell Factories,2022,21. |
| APA | Yang,Qiang.,Zheng,Zhiming.,Zhao,Genhai.,Wang,Li.,Wang,Han.,...&Wang,Peng.(2022).Engineering microbial consortia of Elizabethkingia meningoseptica and Escherichia coli strains for the biosynthesis of vitamin K2.Microbial Cell Factories,21. |
| MLA | Yang,Qiang,et al."Engineering microbial consortia of Elizabethkingia meningoseptica and Escherichia coli strains for the biosynthesis of vitamin K2".Microbial Cell Factories 21(2022). |
入库方式： OAI收割
来源：合肥物质科学研究院
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