Three-dimensional ATUM-SEM reconstruction and analysis of hepatic endoplasmic reticulum-organelle interactions
文献类型：期刊论文
| 作者 | Jiang, Yi2,3 ; Li, Linlin2; Chen, Xi2; Liu, Jiazheng1,2; Yuan, Jingbin2,3; Xie, Qiwei4; Han, Hua1,2,5
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| 刊名 | JOURNAL OF MOLECULAR CELL BIOLOGY
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| 出版日期 | 2021-09-01 |
| 卷号 | 13期号:9页码:636-645 |
| 关键词 | 3D reconstruction ATUM-SEM deep learning liver ER MCSs |
| ISSN号 | 1674-2788 |
| DOI | 10.1093/jmcb/mjab032 |
| 通讯作者 | Han, Hua(hua.han@ia.ac.cn) |
| 英文摘要 | The endoplasmic reticulum (ER) is a contiguous and complicated membrane network in eukaryotic cells, and membrane contact sites (MCSs) between the ER and other organelles perform vital cellular functions, including lipid homeostasis, metabolite exchange, calcium level regulation, and organelle division. Here, we establish a whole pipeline to reconstruct all ER, mitochondria, lipid droplets, lysosomes, peroxisomes, and nuclei by automated tape-collecting ultramicrotome scanning electron microscopy and deep learning techniques, which generates an unprecedented 3D model for mapping liver samples. Furthermore, the morphology of various organelles and the MCSs between the ER and other organelles are systematically analyzed. We found that the ER presents with predominantly flat cisternae and is knitted tightly all throughout the intracellular space and around other organelles. In addition, the ER has a smaller volume-to-membrane surface area ratio than other organelles, which suggests that the ER could be more suited for functions that require a large membrane surface area. Our data also indicate that ER-mitochondria contacts are particularly abundant, especially for branched mitochondria. Our study provides 3D reconstructions of various organelles in liver samples together with important fundamental information for biochemical and functional studies in the liver. |
| WOS关键词 | SCANNING-ELECTRON-MICROSCOPY ; MEMBRANE CONTACT SITES ; MITOCHONDRIA ; ER ; ORGANIZATION ; CELLS |
| 资助项目 | Special Program of Beijing Municipal Science and Technology Commission[Z18110000 3818001] ; Special Program of Beijing Municipal Science and Technology Commission[Z181100000118002] ; Strategic Priority Research Program of Chinese Academy of Science[XDB32030200] ; International Partnership of Chinese Academy of Science[153D31KYSB20170059] |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000733361500003 |
| 出版者 | OXFORD UNIV PRESS |
| 资助机构 | Special Program of Beijing Municipal Science and Technology Commission ; Strategic Priority Research Program of Chinese Academy of Science ; International Partnership of Chinese Academy of Science |
| 源URL | [http://ir.ia.ac.cn/handle/173211/46956]  |
| 专题 | 类脑智能研究中心_微观重建与智能分析 |
| 通讯作者 | Han, Hua |
| 作者单位 | 1.Univ Chinese Acad Sci, Sch Future Technol, Beijing 101408, Peoples R China 2.Chinese Acad Sci, Inst Automat, Natl Lab Pattern Recognit, Beijing 100190, Peoples R China 3.Univ Chinese Acad Sci, Sch Artificial Intelligence, Beijing 101408, Peoples R China 4.Beijing Univ Technol, Data Min Lab, Beijing 100124, Peoples R China 5.CAS Ctr Excellence Brain Sci & Intelligence Techn, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Yi,Li, Linlin,Chen, Xi,et al. Three-dimensional ATUM-SEM reconstruction and analysis of hepatic endoplasmic reticulum-organelle interactions[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2021,13(9):636-645. |
| APA | Jiang, Yi.,Li, Linlin.,Chen, Xi.,Liu, Jiazheng.,Yuan, Jingbin.,...&Han, Hua.(2021).Three-dimensional ATUM-SEM reconstruction and analysis of hepatic endoplasmic reticulum-organelle interactions.JOURNAL OF MOLECULAR CELL BIOLOGY,13(9),636-645. |
| MLA | Jiang, Yi,et al."Three-dimensional ATUM-SEM reconstruction and analysis of hepatic endoplasmic reticulum-organelle interactions".JOURNAL OF MOLECULAR CELL BIOLOGY 13.9(2021):636-645. |
入库方式： OAI收割
来源：自动化研究所
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