UPLC-TOF-MS Method for Simultaneous Quantification of Steroid Hormones in Tissue Homogenates of Zebrafish with Solid-Phase Extraction
文献类型：期刊论文
| 作者 | Li, Yaxi3,4; Yan, Zhi2,4; Li, Xiaodong4; Yin, Xiuli4 ; Li, Ke1,4
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| 刊名 | MOLECULES
 |
| 出版日期 | 2021-10-01 |
| 卷号 | 26期号:20页码:13 |
| 关键词 | quantitative analysis validation steroid hormone stressor behavioral ecology |
| DOI | 10.3390/molecules26206213 |
| 通讯作者 | Li, Ke(kli@yic.ac.cn) |
| 英文摘要 | The quantification of steroid hormones of individual zebrafish (Danio rerio) provides perspective to understand endogenous hormone function. A UPLC-TOF-MS method was developed to provide a reproducible, sensitive, and efficient assay to determine the concentration of steroid hormones, including cortisol, testosterone, androstenedione, 11-deoxycortisol, 11-deoxycorticosterone, and 17-hydroxyprogesterone in whole-body homogenates of each zebrafish. Solid-phase extraction was used to sample matrix clean-up and acquired a recovery from 89.7% to 107.9%. The analytes were separated on an Aquity BEH C18 column using gradient elution. Mass spectrometric analysis was performed by single reaction monitoring (SRM) using positive electrospray ionization mode. The total running time was 6 min, which was greatly shortened compared with a previously reported method. The developed method exhibited excellent linearity for all the analytes, with regression coefficients higher than 0.99. The limit of detection varied between 0.1 and 0.5 ng/L and the limit of quantification was 0.5-1.7 ng/L for all analytes. The precision of the method was assessed on replicate measurements and was found to be in the ranges of 1.9 % to 6.6% and 4.3% to 8.6%, for intra- and inter-day analysis, respectively. This method was validated according to FDA guidance and applied to determine steroid hormone levels in the tissue homogenate of zebrafish acutely treated with caffeine and ethanol. |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; LC-MS/MS ; SALIVARY CORTISOL ; ADULT ZEBRAFISH ; STRESS ; IMMUNOASSAY ; TESTOSTERONE ; GLUCURONIDES ; SENSITIVITY ; RESOLUTION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000716404400001 |
| 资助机构 | Key Deployment Project of the Center for Ocean Mega-Science, Chinese Academy of Sciences ; STS Program of CAS ; National Overseas High-level Talent Project ; Taishan Scholar Program from Shandong Province of China ; Shuangbai Plan from Yantai Municipal City |
| 源URL | [http://ir.yic.ac.cn/handle/133337/30735]  |
| 专题 | 海岸带生物资源高效利用研究与发展中心 支撑部门_所级公共技术服务中心 |
| 通讯作者 | Li, Ke |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 2.Yantai Univ, Sch Ocean, Yantai 264005, Peoples R China 3.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yaxi,Yan, Zhi,Li, Xiaodong,et al. UPLC-TOF-MS Method for Simultaneous Quantification of Steroid Hormones in Tissue Homogenates of Zebrafish with Solid-Phase Extraction[J]. MOLECULES,2021,26(20):13. |
| APA | Li, Yaxi,Yan, Zhi,Li, Xiaodong,Yin, Xiuli,&Li, Ke.(2021).UPLC-TOF-MS Method for Simultaneous Quantification of Steroid Hormones in Tissue Homogenates of Zebrafish with Solid-Phase Extraction.MOLECULES,26(20),13. |
| MLA | Li, Yaxi,et al."UPLC-TOF-MS Method for Simultaneous Quantification of Steroid Hormones in Tissue Homogenates of Zebrafish with Solid-Phase Extraction".MOLECULES 26.20(2021):13. |
入库方式： OAI收割
来源：烟台海岸带研究所
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