A rapid and simple signature peptides-based method for species authentication of three main commercial Pheretima
文献类型：期刊论文
| 作者 | Liu, Qiao2; Bi, Qirui2,3; Zhang, Jingxian1; Qin, Weiwei2; Yi, Shanyong2; Hu, Qing1; Sun, Jian1; Ji, Shen1; Tan, Ninghua2 |
| 刊名 | JOURNAL OF PROTEOMICS
 |
| 出版日期 | 2022-03-20 |
| 卷号 | 255页码:7 |
| 关键词 | Pheretima Species authentication Proteomics Signature peptide Multiple reaction monitoring |
| ISSN号 | 1874-3919 |
| DOI | 10.1016/j.jprot.2021.104456 |
| 通讯作者 | Ji, Shen(jishen2013@163.com) ; Tan, Ninghua(nhtan@cpu.edu.cn) |
| 英文摘要 | Pheretima with various activities is a commonly used animal-derived traditional medicine in Asia countries. However, almost half of them are non-pharmacopoeia species in the market due to the similar morphological characteristics between medicinal and non-medicinal species. This study aims to establish an effective method based on signature peptides for species authentication of three main commercial Pheretima, including two major Pheretima species (Amynthas aspergillum, Metaphire vulgaris) and one main adulteration (Metaphire magna). Firstly, the species of 52 batches of commercial Pheretima were authenticated based on DNA barcodes. Secondly, proteomic analysis was performed for protein characterization of three main commercial Pheretima. Furthermore, their signature peptides were screened and validated using ultra-high performance liquid chromatography coupled with mass spectrometry (UPLC-MS/MS) in multiple reaction monitoring (MRM) mode. Moreover, a simplified sample processing method was developed. Finally, large quantities of commercial Pheretima samples were analyzed for further verifying the feasibility of the signature peptides-based method. The result showed that the established method had a great application potential for authenticity identification of commercial Pheretima. Significance: The authenticity assessment of medicinal materials is a main issue in the quality control process as deceptive practices could imply severe health risks. In this study, a rapid and simple method based on signature peptides was established for species authentication of three main commercial Pheretima, which can be an effective alternative to complex DNA barcoding and difficult morphological identification, and provided a reference for improvement of Pheretima quality standards. |
| WOS关键词 | IDENTIFICATION ; PROTEINS |
| 资助项目 | National Science Foundation for Young Scientists of China[81803716] ; Science Foundation of Youths of Jiangsu Province of China[BK 20170738] ; Research Fund for Post Doctorate of Jiangsu Province of China[1701055A] ; the Double First-Class University Project of China Pharmaceutical University[CPU2018GF05] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000787266500010 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/299517]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Ji, Shen; Tan, Ninghua |
| 作者单位 | 1.Shanghai Inst Food & Drug Control, NMPA Key Lab Qual Control Tradit Chinese Med, 1500 Zhangheng Rd, Shanghai 201203, Peoples R China 2.China Pharmaceut Univ, Sch Tradit Chinese Pharm, Dept TCMs Pharmaceut, Nanjing 211198, Peoples R China 3.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Qiao,Bi, Qirui,Zhang, Jingxian,et al. A rapid and simple signature peptides-based method for species authentication of three main commercial Pheretima[J]. JOURNAL OF PROTEOMICS,2022,255:7. |
| APA | Liu, Qiao.,Bi, Qirui.,Zhang, Jingxian.,Qin, Weiwei.,Yi, Shanyong.,...&Tan, Ninghua.(2022).A rapid and simple signature peptides-based method for species authentication of three main commercial Pheretima.JOURNAL OF PROTEOMICS,255,7. |
| MLA | Liu, Qiao,et al."A rapid and simple signature peptides-based method for species authentication of three main commercial Pheretima".JOURNAL OF PROTEOMICS 255(2022):7. |
入库方式： OAI收割
来源：上海药物研究所
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