Integrated proteome and phosphoproteome analysis of interscapular brown adipose and subcutaneous white adipose tissues upon high fat diet feeding in mouse
文献类型:期刊论文
作者 | Wang, Yuqiu2,3; Li, Junmin1; Lu, Dayun3; Meng, Qian3; Song, Nixue3; Zhou, Hu3; Xiao, Xiao2; Sun, Liming4; Zhu, Hongwen3 |
刊名 | JOURNAL OF PROTEOMICS |
出版日期 | 2022-03-20 |
卷号 | 255页码:13 |
ISSN号 | 1874-3919 |
关键词 | Brown adipose tissue Subcutaneous white adipose tissue High fat diet Proteomics Phosphoproteomics Metabolism |
DOI | 10.1016/j.jprot.2022.104500 |
通讯作者 | Xiao, Xiao(xiaoxiao@ecust.edu.cn) ; Sun, Liming(sunlm@ucas.ac.cn) ; Zhu, Hongwen(zhw@simm.ac.cn) |
英文摘要 | Thermogenesis is a promising approach to limit weight gain in response to excess nutrition. In contrast to cold induced thermogenesis, the molecular and cellular mechanisms of diet-induced thermogenesis (DIT) have not been fully characterized. Here, we explored the response of brown adipose tissue (BAT) and subcutaneous white adipose tissue (sWAT) to high fat diet (HFD) using proteome and phosphoproteome analysis. We observed that after HFD, Uncoupling protein 1 (UCP1) and its phosphorylation were only increased in BAT. Furthermore, proteins involved in fatty acid oxidation, tricarboxylic acid cycle, and oxidative phosphorylation were also upregulated in BAT. Nevertheless, most metabolic related proteins were downregulated in sWAT. We found that these metabolic changes accompanied with different variation of mitochondrial proteins between BAT and sWAT. After HFD, most mitochondrial proteins were decreased in sWAT, but not in BAT. This effect was correlated with decreased mitochondrial ribosomal proteins in sWAT. Finally, through phosphoproteomic analysis, we predicted the activities of kinases in HFD mice and observed that there were more kinases inactivated in sWAT. Finally, this dataset provides a valuable resource for molecular researchers in the fields of obesity and obesity-related disease. Significance: Thermogenesis is a promising approach to combat obesity in response to excess energy. Nevertheless, the molecular and cellular mechanisms of DIT have not been fully characterized. Herein, we employed mass spectrometry (MS)-based proteomics and phosphoproteomics to identify differentially regulated proteins and phosphosites in BAT and sWAT of mice fed with HFD. This study unveils the differential regulatory networks of HFD in BAT and sWAT, which provides reference omics data to future researchers. |
WOS关键词 | INDUCED THERMOGENESIS ; INSULIN-RESISTANCE ; BLOOD-FLOW ; OBESITY ; BEIGE ; MICE ; GENE ; ACTIVATION ; EXPRESSION ; LIPOLYSIS |
资助项目 | Yangfan Project of Shanghai Science and Technology Commission[20YF1457300] |
WOS研究方向 | Biochemistry & Molecular Biology |
语种 | 英语 |
出版者 | ELSEVIER |
WOS记录号 | WOS:000787266500006 |
源URL | [http://119.78.100.183/handle/2S10ELR8/299520] |
专题 | 新药研究国家重点实验室 |
通讯作者 | Xiao, Xiao; Sun, Liming; Zhu, Hongwen |
作者单位 | 1.Fudan Univ, Zhongshan Hosp, Surg Crit Care Med, Xuhui Branch, Shanghai, Peoples R China 2.East China Univ Sci & Technol, Sch Bioengn, Shanghai 200237, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, State Key Lab Drug Res, Shanghai 201203, Peoples R China 4.Univ Chinese Acad Sci, Sch Life Sci, Hangzhou Inst Adv Study, Hangzhou 310024, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Yuqiu,Li, Junmin,Lu, Dayun,et al. Integrated proteome and phosphoproteome analysis of interscapular brown adipose and subcutaneous white adipose tissues upon high fat diet feeding in mouse[J]. JOURNAL OF PROTEOMICS,2022,255:13. |
APA | Wang, Yuqiu.,Li, Junmin.,Lu, Dayun.,Meng, Qian.,Song, Nixue.,...&Zhu, Hongwen.(2022).Integrated proteome and phosphoproteome analysis of interscapular brown adipose and subcutaneous white adipose tissues upon high fat diet feeding in mouse.JOURNAL OF PROTEOMICS,255,13. |
MLA | Wang, Yuqiu,et al."Integrated proteome and phosphoproteome analysis of interscapular brown adipose and subcutaneous white adipose tissues upon high fat diet feeding in mouse".JOURNAL OF PROTEOMICS 255(2022):13. |
入库方式: OAI收割
来源:上海药物研究所
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