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Neutralization of SARS-CoV-2 pseudovirus using ACE2-engineered extracellular vesicles

文献类型：期刊论文

作者Wu, Canhao2,6; Xu, Qin6; Wang, Huiyuan2; Tu, Bin2,3; Zeng, Jiaxin2,6; Zhao, Pengfei2,5; Shi, Mingjie2; Qiu, Hong2; Huang, Yongzhuo1,2,3,4
刊名ACTA PHARMACEUTICA SINICA B
出版日期2022-03-01
卷号12期号:3页码:1523-1533
关键词SARS-CoV-2 COVID-19 Spike protein Pseudovirus Extracellular vesicles ACE2 Intranasal administration Neutralization
ISSN号2211-3835
DOI10.1016/j.apsb.2021.09.004
通讯作者Wang, Huiyuan(wanghuiyuan@simm.ac.cn) ; Qiu, Hong(hongqiu@simm.ac.cn) ; Huang, Yongzhuo(yzhuang@simm.ac.cn)
英文摘要The spread of coronavirus disease 2019 (COVID-19) throughout the world has resulted in stressful healthcare burdens and global health crises. Developing an effective measure to protect people from infection is an urgent need. The blockage of interaction between angiotensin-converting enzyme 2 (ACE2) and S protein is considered an essential target for anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) drugs. A full-length ACE2 protein could be a potential drug to block early entry of SARS-CoV-2 into host cells. In this study, a therapeutic strategy was developed by using extracellular vesicles (EVs) with decoy receptor ACE2 for neutralization of SARS-CoV-2. The EVs embedded with engineered ACE2 (EVs-ACE2) were prepared; the EVs-ACE2 were derived from an engineered cell line with stable ACE2 expression. The potential effect of the EVs-ACE2 on anti-SARS-CoV-2 was demonstrated by both in vitro and in vivo neutralization experiments using the pseudovirus with the S protein (S-pseudovirus). EVs-ACE2 can inhibit the infection of S-pseudovirus in various cells, and importantly, the mice treated with intranasal administration of EVs-ACE2 can suppress the entry of S-pseudovirus into the mucosal epithelium. Therefore, the intranasal EVs-ACE2 could be a preventive medicine to protect from SARS-CoV-2 infection. This EVs-based strategy offers a potential route to COVID-19 drug development. (C) 2022 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V.
WOS关键词COVID-19 ; EXOSOMES ; CANCER ; ACE2
资助项目National Special Project for Significant Drugs Development (China)[2018ZX09711002-010-002] ; National Natural Science Foundation of China (China)[81925035] ; National Natural Science Foundation of China (China)[81521005] ; Shanghai Sci-Tech Innovation Initiative (China)[19431903100] ; Shanghai Sci-Tech Innovation Initiative (China)[18430740800] ; Shanghai Collaborative Innovation Group of Early Diagnosis and Precise Treatment of Hemangiomas (China)[SSMU-ZDCX20180701] ; Sanofi-SIBS Yong Faculty Award ; Youth Innovation Promotion Association
WOS研究方向Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000782810600007
出版者INST MATERIA MEDICA, CHINESE ACAD MEDICAL SCIENCES
源URL[http://119.78.100.183/handle/2S10ELR8/299743]  
专题新药研究国家重点实验室
通讯作者Wang, Huiyuan; Qiu, Hong; Huang, Yongzhuo
作者单位1.Taizhou Univ, Sch Adv Study, Inst Nat Med & Hlth Prod, Taizhou 318000, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
3.Chinese Acad Sci, Zhongshan Inst Drug Discovery, SIMM, Zhongshan 528437, Peoples R China
4.NMPA Key Lab Qual Res & Evaluat Pharmaceut Excipi, Shanghai 201203, Peoples R China
5.Zhejiang Univ, Ctr Clin Pharmacol, Sch Med, Affiliated Hosp 2, Hangzhou 310009, Peoples R China
6.Guangzhou Univ Chinese Med, Artemisinin Res Ctr, Clin Sch 1, Guangzhou 510450, Peoples R China
推荐引用方式
GB/T 7714		 Wu, Canhao,Xu, Qin,Wang, Huiyuan,et al. Neutralization of SARS-CoV-2 pseudovirus using ACE2-engineered extracellular vesicles[J]. ACTA PHARMACEUTICA SINICA B,2022,12(3):1523-1533.
APA Wu, Canhao.,Xu, Qin.,Wang, Huiyuan.,Tu, Bin.,Zeng, Jiaxin.,...&Huang, Yongzhuo.(2022).Neutralization of SARS-CoV-2 pseudovirus using ACE2-engineered extracellular vesicles.ACTA PHARMACEUTICA SINICA B,12(3),1523-1533.
MLA Wu, Canhao,et al."Neutralization of SARS-CoV-2 pseudovirus using ACE2-engineered extracellular vesicles".ACTA PHARMACEUTICA SINICA B 12.3(2022):1523-1533.

入库方式： OAI收割

来源：上海药物研究所

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