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Development and validation of a liquid chromeatography-tandem mass spectrometry method for simultaneous quantification of medium- and long-chain saturated fatty acids in hamster plasma samples

文献类型：期刊论文

作者Li, Fan2,3; Zhang, Qingli3; Tong, Yue1,3; Jiang, Jianlan2; Liu, Jia3,4
刊名RAPID COMMUNICATIONS IN MASS SPECTROMETRY
出版日期2022-06-15
卷号36期号:11页码:11
ISSN号0951-4198
DOI10.1002/rcm.9280
通讯作者Jiang, Jianlan(jljiang@tju.edu.cn) ; Liu, Jia(jia.liu@simm.ac.cn)
英文摘要Rationale Saturated fatty acids (SFAs) are associated with many diseases in humans. Developing a reliable analytical method to analyze SFAs in plasma is essential to understand their biological activities. An ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC/MS/MS) method has been developed for the quantification of medium- and long-chain SFAs (M/LCSFAs) in hamster plasma. Methods We compared three methods (DOLE, Folch and MTBE) for extracting M/LCSFAs from plasma. The M/LCSFA derivatives were separated using a C-18 column. The method was validated and applied to analyze M/LCSFA concentrations in normal-fat diet (NFD) and high-fat diet (HFD) hamster plasma. Results Among the three extraction methods, the DOLE method had the highest extraction recovery and was simple to operate with a short incubation time. All of the calibration curves exhibited good linear relationships (r >= 0.9958). The results for selectivity, accuracy, precision, matrix effects and recovery were all within the acceptance criteria. In HFD hamster plasma, the concentration of M/LCSFAs with even-carbon chain length was significantly increased. Conclusions A simple, robust and reproducible method for the simultaneous quantification of M/LCSFAs by UHPLC/MS/MS was developed and validated. The method gave successfully quantification of M/LCSFAs in plasma samples from NFD and HFD hamsters.
WOS关键词INSULIN-RESISTANCE ; HUMAN BLOOD ; QUANTITATION ; SERUM ; EXTRACTION ; ASSAY
资助项目Shanghai Municipal Science and Technology Major Project ; Key-Area Research and Development Program of Guangdong Province[2020B0303070002] ; Science and Technology Commission of Shanghai Municipality[20430780300] ; National Natural Science Foundation of China[21920102003] ; National Natural Science Foundation of China[81803610]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy
语种英语
WOS记录号WOS:000773091300001
出版者WILEY
源URL[http://119.78.100.183/handle/2S10ELR8/299909]  
专题中国科学院上海药物研究所
通讯作者Jiang, Jianlan; Liu, Jia
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China
2.Tianjin Univ, Sch Chem Engn & Technol, Minist Educ, Key Lab Syst Bioengn, Tianjin, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China
4.Univ Chinese Acad Sci, Sch Pharmaceut Sci & Technol, Hangzhou Inst Adv Study, Hangzhou, Peoples R China
推荐引用方式
GB/T 7714		 Li, Fan,Zhang, Qingli,Tong, Yue,et al. Development and validation of a liquid chromeatography-tandem mass spectrometry method for simultaneous quantification of medium- and long-chain saturated fatty acids in hamster plasma samples[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2022,36(11):11.
APA Li, Fan,Zhang, Qingli,Tong, Yue,Jiang, Jianlan,&Liu, Jia.(2022).Development and validation of a liquid chromeatography-tandem mass spectrometry method for simultaneous quantification of medium- and long-chain saturated fatty acids in hamster plasma samples.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,36(11),11.
MLA Li, Fan,et al."Development and validation of a liquid chromeatography-tandem mass spectrometry method for simultaneous quantification of medium- and long-chain saturated fatty acids in hamster plasma samples".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 36.11(2022):11.

入库方式： OAI收割

来源：上海药物研究所

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