Endo-M Mediated Chemoenzymatic Approach Enables Reversible Glycopeptide Labeling for O-GlcNAcylation Analysis
文献类型：期刊论文
| 作者 | Chen, Yao2,3; Tang, Feng3,4; Qin, Hongqiang2,3; Yue, Xuyang2; Nie, Yongzhan1,5; Huang, Wei3,4; Ye, Mingliang2,3 |
| 刊名 | ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
 |
| 出版日期 | 2022-03-30 |
| 页码 | 9 |
| ISSN号 | 1433-7851 |
| 关键词 | Chemoenzymatic Labeling Glycopeptide Enrichment Glycoproteomics Hydrophilic Interaction Liquid Chromatography O-GlcNAcylation |
| DOI | 10.1002/anie.202117849 |
| 通讯作者 | Qin, Hongqiang(qinhq@dicp.ac.cn) ; Huang, Wei(huangwei@simm.ac.cn) ; Ye, Mingliang(mingliang@dicp.ac.cn) |
| 英文摘要 | To selectively enrich O-linked beta-N-acetylglucosamine (O-GlcNAc) peptides in their original form from complex samples, we report the first reversible chemoenzymatic labeling approach for proteomic analysis. In this strategy, the O-GlcNAc moieties are ligated with long N-glycans using an Endo-M mutant, which enables the enrichment of the labeled glycopeptides by hydrophilic interaction liquid chromatography (HILIC). The attached glycans on the enriched glycopeptides are removed by wild-type Endo-M/S to restore the O-GlcNAc moiety. Compared with classic chemoenzymatic labeling, this approach enables the tag-free identification, and eliminates the interference of bulky tags in glycopeptide detection. This approach presents a unique avenue for the proteome-wide analysis of protein O-GlcNAcylation to promote its mechanism research. |
| WOS关键词 | BETA-N-ACETYLGLUCOSAMINE ; MODIFIED PROTEINS ; CROSS-TALK ; GLYCOSYLATION ; PHOSPHORYLATION ; TRANSCRIPTION ; DYNAMICS ; STRESS |
| 资助项目 | National Natural Science Foundation of China[81730016] ; National Natural Science Foundation of China[21775146] ; National Natural Science Foundation of China[22034007] ; National Key Research and Development Program of China[2017YFA0505003] ; National Key Research and Development Program of China[2020YFE0202200] ; Innovation Program of Science and Research from the DICP, CAS[DICP I201919] ; Innovation Program of Science and Research from the DICP, CAS[DICPQIBEBT UN201802] ; Youth Innovation Promotion Association of CAS[2018212] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| WOS记录号 | WOS:000775116900001 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/299960]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Qin, Hongqiang; Huang, Wei; Ye, Mingliang |
| 作者单位 | 1.Fourth Mil Med Univ, Natl Clin Res Ctr Digest Dis, State Key Lab Canc Biol, Xian 710032, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Univ Chinese Acad Sci, Beijing 101408, Peoples R China 4.Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Ctr Biotherapeut Discovery Res, Shanghai Inst Mat Med,CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China 5.Fourth Mil Med Univ, Xijing Hosp Digest Dis, Xian 710032, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Yao,Tang, Feng,Qin, Hongqiang,et al. Endo-M Mediated Chemoenzymatic Approach Enables Reversible Glycopeptide Labeling for O-GlcNAcylation Analysis[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2022:9. |
| APA | Chen, Yao.,Tang, Feng.,Qin, Hongqiang.,Yue, Xuyang.,Nie, Yongzhan.,...&Ye, Mingliang.(2022).Endo-M Mediated Chemoenzymatic Approach Enables Reversible Glycopeptide Labeling for O-GlcNAcylation Analysis.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,9. |
| MLA | Chen, Yao,et al."Endo-M Mediated Chemoenzymatic Approach Enables Reversible Glycopeptide Labeling for O-GlcNAcylation Analysis".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2022):9. |
入库方式： OAI收割
来源：上海药物研究所
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