Comprehensive analysis of natural polysaccharides from TCMs: a generic approach based on UPLC-MS/MS
文献类型：期刊论文
| 作者 | Wang, Tian-Long2,3; Li, Yi-Cong1; Lin, Chun-Sheng4; Zou, Yi-Ping1 |
| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2022-02-01 |
| 卷号 | 277页码:11 |
| 关键词 | Polysaccharides UHPLC-ESI-MS Aldononitrile acetates Compositional analysis |
| ISSN号 | 0144-8617 |
| DOI | 10.1016/j.carbpol.2021.118877 |
| 通讯作者 | Zou, Yi-Ping(zyping66@163.com) |
| 英文摘要 | Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESIMS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min. The separation was performed on a Cortecs C18 column using acetonitrile (A) and water (B) as the mobile phase with gradient elution. The target components were detected in selected ion monitoring (SIM) mode by mass spectrometry with an electrospray ionization (ESI) source operating in positive ionization mode. A comparison with traditional methods was used to determine the validity of the results. The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides. The results demonstrate that this protocol is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from TCMs. |
| WOS关键词 | MONOSACCHARIDE COMPOSITION ANALYSIS ; ANION-EXCHANGE CHROMATOGRAPHY ; MASS-SPECTROMETRIC CHARACTERIZATION ; GAS-CHROMATOGRAPHY ; LC-MS ; ANTIINFLAMMATORY ACTIVITY ; ANTITUMOR POLYSACCHARIDE ; ANTIOXIDANT ACTIVITIES ; LIQUID-CHROMATOGRAPHY ; ACETATE DERIVATIVES |
| 资助项目 | Postdoctoral Research Foundation of China[2020067] ; Collaborative Innovation Center for Modern Science and Technology and Industrial Development of Jiangxi Traditional Medicine[2020YBBGWL010] ; 5511 Science and Technology Innovation Talent Project of Jiangxi Province[2019YCKJ5511PK29] |
| WOS研究方向 | Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000761128400012 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/300185]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zou, Yi-Ping |
| 作者单位 | 1.Yichun Univ, Jiangxi Key Lab Act Ingredients Nat Drugs, Yichun 336000, Peoples R China 2.Guangdong Macau Tradit Chinese Med Technol Ind Pk, Zhuhai 519000, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201210, Peoples R China 4.Heilongjiang Univ Chinese Med, Affiliated Hosp 2, Harbin 150001, Heilongjiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Tian-Long,Li, Yi-Cong,Lin, Chun-Sheng,et al. Comprehensive analysis of natural polysaccharides from TCMs: a generic approach based on UPLC-MS/MS[J]. CARBOHYDRATE POLYMERS,2022,277:11. |
| APA | Wang, Tian-Long,Li, Yi-Cong,Lin, Chun-Sheng,&Zou, Yi-Ping.(2022).Comprehensive analysis of natural polysaccharides from TCMs: a generic approach based on UPLC-MS/MS.CARBOHYDRATE POLYMERS,277,11. |
| MLA | Wang, Tian-Long,et al."Comprehensive analysis of natural polysaccharides from TCMs: a generic approach based on UPLC-MS/MS".CARBOHYDRATE POLYMERS 277(2022):11. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。