Deep 2-Hydroxyisobutyrylome in mouse liver expands the roles of lysine 2-hydroxyisobutyrylation pathway
文献类型：期刊论文
| 作者 | Du, Runhua1,2; Liu, Guobin1,3; Huang, He1,2,3 |
| 刊名 | BIOORGANIC & MEDICINAL CHEMISTRY
 |
| 出版日期 | 2022-03-01 |
| 卷号 | 57页码:7 |
| 关键词 | Lysine 2-hydroxyisobutyrylation Post-translational modification Proteomics Differential expression |
| ISSN号 | 0968-0896 |
| DOI | 10.1016/j.bmc.2022.116634 |
| 通讯作者 | Huang, He(hhuang@simm.ac.cn) |
| 英文摘要 | Lysine 2-hydroxyisobutyrylation (Khib), a newly characterized post-translational modification, is conserved in both eukaryotic and prokaryotic cells. At present, only about 6500 Khib sites have been identified in mammalian cells, which is insufficient compared with the well-known acetylation and thus hinders the understanding of its roles in diverse cellular processes. Here, utilizing immunoaffinity enrichment coupled with LC-MS/MS approach, we carried out a deep proteomics analysis of Khib in mouse liver. A total of 20861 Khib sites in 3768 proteins were identified, which expands the known Khib sites by two folds and represents the deepest Khib proteome in mammalian cells currently. Bioinformatics analysis showed that the 2-hydroxyisobutyrylated proteins have different subcellular localizations and participate in a wide range of molecular functions and cellular processes, such as metabolic processes and disease-related pathways. In addition, RNA-Seq analysis revealed that 1470 genes up-regulated and 790 genes down-regulated in response to elevated Khib levels in HeLa cells. The 1470 up regulated genes were mainly associated with human papillomavirus infection, ECM-receptor interaction, as well as protein digestion and absorption, while the 790 down-regulated genes were mainly enriched in the multiple diseases and Glycolysis/Gluconeogenesis processes. Taken together, our research largely expands the known Khib sites, which helps delineate the biological functions of the Khib pathway and provides mechanistic insights into how Khib exerts its functions in specific cellular pathways. |
| WOS关键词 | GENE-EXPRESSION ; METABOLIC-REGULATION ; KAISO ; P120(CTN) ; CLUSTERIN ; PACKAGE ; PROTEIN |
| 资助项目 | Shanghai Municipal Science and Technology Major Project |
| WOS研究方向 | Biochemistry & Molecular Biology ; Pharmacology & Pharmacy ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000765951500003 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/300233]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Huang, He |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Runhua,Liu, Guobin,Huang, He. Deep 2-Hydroxyisobutyrylome in mouse liver expands the roles of lysine 2-hydroxyisobutyrylation pathway[J]. BIOORGANIC & MEDICINAL CHEMISTRY,2022,57:7. |
| APA | Du, Runhua,Liu, Guobin,&Huang, He.(2022).Deep 2-Hydroxyisobutyrylome in mouse liver expands the roles of lysine 2-hydroxyisobutyrylation pathway.BIOORGANIC & MEDICINAL CHEMISTRY,57,7. |
| MLA | Du, Runhua,et al."Deep 2-Hydroxyisobutyrylome in mouse liver expands the roles of lysine 2-hydroxyisobutyrylation pathway".BIOORGANIC & MEDICINAL CHEMISTRY 57(2022):7. |
入库方式： OAI收割
来源：上海药物研究所
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