中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 新药研究国家重点实验室
Tumour-derived extracellular vesicle membrane hybrid lipid nanovesicles enhance siRNA delivery by tumour-homing and intracellular freeway transportation

文献类型：期刊论文

作者Zhou, Xin1; Miao, Yunqiu1; Wang, Ying1,2; He, Shufang1; Guo, Linmiao1,2; Mao, Junsong1; Chen, Mingshu1; Yang, Yuting1; Zhang, Xinxin1,2; Gan, Yong1,2
刊名JOURNAL OF EXTRACELLULAR VESICLES
出版日期2022-03-01
卷号11期号:3页码:16
关键词tumour-derived extracellular vesicles hybrid lipid nanovesicles tumour homing siRNA delivery hepatocellular carcinoma
DOI10.1002/jev2.12198
通讯作者Zhang, Xinxin(xinxinzheng@simm.ac.cn) ; Gan, Yong(ygan@simm.ac.cn)
英文摘要Extracellular vesicles (EVs) have been proved a promising small interfering RNA (siRNA) delivery vehicle to mediate gene-silencing. Tumour-derived extracellular vesicles (TDEVs) as genetic exchange vectors in the tumour microenvironment, enable intercellular communication for a wide range of endogenous cargo molecules, such as RNAs and proteins. However, the oncogenic cargo of TDEVs limits their application in siRNA delivery for cancer therapy. Herein, we isolated TDEVs from hepatocellular carcinoma (HCC) cells and derived TDEV membranes by abandoning their content. Innovative TDEV membrane hybrid lipid nanovesicles (LEVs) were then fabricated by fusion of TDEV membranes and phospholipids to realize precise delivery to tumours and highly efficient transfection of siRNA. The TDEV membranes endow LEVs with 'homing' targeting ability, facilitating specific internalisation into parent HCC cells primarily through heparan sulfate proteoglycan-mediated pathways. Unlike conventional lipid-based nanovesicles, LEVs can bypass the endosomal degradation pathway, boost the delivery of siRNA through the Golgi and endoplasmic reticulum (ER) intracellular 'freeway' transportation, achieving a 1.7-fold improvement in siRNA transfection efficiency compared with liposomes. Additionally, siRNA loaded LEVs were demonstrated to enhance the antitumour efficacy in HCC bearing mice through effective gene silencing in the tumour sites. Our results highlight the potential application of the TDEV membrane-derived nanovesicles as an advanced siRNA delivery strategy for cancer therapy.
WOS关键词THERAPY ; SURFACE ; BRAIN
资助项目National Natural Science Foundation of China[81973250] ; National Natural Science Foundation of China[82025032] ; Natural Science Foundation of Shanghai[21ZR1475800] ; State Key Laboratory of Drug Research[SIMM2103ZZ-01] ; Major International Joint Research Project of Chinese Academy of Sciences[153631KYSB20190020]
WOS研究方向Cell Biology
语种英语
WOS记录号WOS:000762511800001
出版者WILEY
源URL[http://119.78.100.183/handle/2S10ELR8/300309]  
专题新药研究国家重点实验室
通讯作者Zhang, Xinxin; Gan, Yong
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, 501 Haike Rd, Shanghai 201203, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Zhou, Xin,Miao, Yunqiu,Wang, Ying,et al. Tumour-derived extracellular vesicle membrane hybrid lipid nanovesicles enhance siRNA delivery by tumour-homing and intracellular freeway transportation[J]. JOURNAL OF EXTRACELLULAR VESICLES,2022,11(3):16.
APA Zhou, Xin.,Miao, Yunqiu.,Wang, Ying.,He, Shufang.,Guo, Linmiao.,...&Gan, Yong.(2022).Tumour-derived extracellular vesicle membrane hybrid lipid nanovesicles enhance siRNA delivery by tumour-homing and intracellular freeway transportation.JOURNAL OF EXTRACELLULAR VESICLES,11(3),16.
MLA Zhou, Xin,et al."Tumour-derived extracellular vesicle membrane hybrid lipid nanovesicles enhance siRNA delivery by tumour-homing and intracellular freeway transportation".JOURNAL OF EXTRACELLULAR VESICLES 11.3(2022):16.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。